{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,11]],"date-time":"2023-10-11T05:40:32Z","timestamp":1697002832273},"reference-count":39,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2006,8,2]],"date-time":"2006-08-02T00:00:00Z","timestamp":1154476800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pediatric Transplantation"],"published-print":{"date-parts":[[2006,11]]},"abstract":"<jats:p><jats:bold>Abstract:\u2002<\/jats:bold> Allogeneic SCT remains the only means of cure for many patients with various malignant disorders as well as non\u2010malignant diseases. Infection together with severe aGvHD may result in a significant incidence of transplant\u2010related morbidity and mortality. Current evidence suggests that hSPS represent major immunodominant antigens in many pathogens and therefore might play an important role in the pathogenesis of GvHD. We investigated the levels of total Ig, IgG and IgM isotype antibodies to rh\u2010hsp60, recombinant <jats:italic>Mycobacterium bovis<\/jats:italic> hsp65 and stress\u2010inducible rh\u2010hsp70 in sera of pediatric patients undergoing SCT by using ELISA. We studied whether humoral immune responses to hSPS follow transplant\u2010related complications, bacterial and fungal infection. Anti\u2010hsp antibodies were detected in patients\u2019 sera before conditioning, over the course of conditioning and all the time post\u2010transplant. We found no correlation between anti\u2010hsp antibodies and the occurrence and severity of GvHD and\/or other transplant\u2010related complications like graft failure, hemorrhagic cystitis and capillary leakage syndrome. However, elevated anti\u2010hsp antibodies involving IgM and IgG isotypes were found to be associated with bacterial and fungal infection depending on etiological agents. We demonstrated de novo humoral response to hSPS in a cohort of patients with actual infection caused by <jats:italic>Klebsiella pneumoniae<\/jats:italic> (anti\u2010hsp60, anti\u2010hsp65 and anti\u2010hsp70), <jats:italic>Pseudomonas aeruginosa<\/jats:italic> (anti\u2010hsp60, anti\u2010hsp70) and <jats:italic>Aspergillus fumigatus<\/jats:italic> (anti\u2010hsp65). We conclude that anti\u2010hsp antibodies might be produced after SCT in relation to infection depending on etiological agents; however, transplant\u2010related complications by themselves had a little impact.<\/jats:p>","DOI":"10.1111\/j.1399-3046.2006.00566.x","type":"journal-article","created":{"date-parts":[[2006,8,2]],"date-time":"2006-08-02T20:08:07Z","timestamp":1154549287000},"page":"794-804","source":"Crossref","is-referenced-by-count":4,"title":["Antibodies to 60, 65 and 70\u2003kDa heat shock proteins in pediatric allogeneic stem cell transplant 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