{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T22:36:13Z","timestamp":1790894173385,"version":"4.1.0"},"reference-count":19,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2004,6,29]],"date-time":"2004-06-29T00:00:00Z","timestamp":1088467200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Xenotransplantation"],"published-print":{"date-parts":[[2004,9]]},"abstract":"<jats:p><jats:bold>Abstract:\u2002<\/jats:bold><jats:bold> Background:\u2002<\/jats:bold> The impact of anti\u2010Gal<jats:italic>\u03b1<\/jats:italic>1\u20103Gal (<jats:italic>\u03b1<\/jats:italic>Gal) antibodies on the acute humoral xenograft rejection (AHXR) of pig organs transplanted in baboons is unclear.<\/jats:p><jats:p><jats:bold>Methods:\u2002<\/jats:bold> Twenty\u2010three baboons underwent heterotopic pig heart transplantation (Tx). Groups A (n\u2003=\u20035) and B (n\u2003=\u20036) received non\u2010transgenic and human decay accelerating factor (hDAF) pig hearts, respectively, without any treatment. Groups C (n\u2003=\u20035) and D (n\u2003=\u20037) were transplanted with non\u2010transgenic and hDAF organs, respectively, and the exclusive treatment was repeated extracorporeal immunoadsorptions (EIA) before and after Tx with an <jats:italic>\u03b1<\/jats:italic>Gal column containing disaccharide (DI), trisaccharide (TRI) 2 and pentasaccharide (PENTA) oligosaccharides.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> In group A, 3 of 5 xenografts underwent hyperacute rejection (HAR). No xenograft from groups B, C and D experienced HAR, most of them failing from AHXR. Immediately after Tx and up to day 2, the level of immunoglobulin (Ig)M and IgG anti\u2010<jats:italic>\u03b1<\/jats:italic>Gal DI, TRI2 and TRI6, and anti\u2010pig hemolytic antibody (APHA) antibodies decreased in all the groups by 80 to 96% compared with the concentration present before Tx. From day 3 to AHXR, a sustained increase of anti\u2010<jats:italic>\u03b1<\/jats:italic>Gal IgM DI, TRI2 and TRI6, and APHA occurred in all groups. EIA depleted anti\u2010<jats:italic>\u03b1<\/jats:italic>Gal IgM and APHA before Tx, but it did not modify the increase of these antibodies after Tx. Baboon serum samples before Tx, pre\u2010incubated in vitro with 1\u2003mg\/ml of DI, TRI2 and TRI6, had an average of 93% reduction of anti\u2010<jats:italic>\u03b1<\/jats:italic>Gal IgM antibodies specific against each one of these <jats:italic>\u03b1<\/jats:italic>Gal oligosaccharides. In contrast, at AHXR, the average reduction after in vitro pre\u2010incubation with either 1 or 5\u2003mg\/ml of DI, TRI2 and TRI6 was 40%.<\/jats:p><jats:p><jats:bold>Conclusions:\u2002<\/jats:bold> The EIA reduces anti\u2010<jats:italic>\u03b1<\/jats:italic>Gal and APHA antibodies, preventing the HAR of non\u2010transgenic pig hearts transplanted in baboons, as does hDAF expression. However, EIA does not modify the level of anti\u2010<jats:italic>\u03b1<\/jats:italic>Gal IgM and APHA antibodies after Tx nor the AHXR of either non\u2010transgenic or hDAF pig organs. The increase in anti\u2010<jats:italic>\u03b1<\/jats:italic>Gal IgM after Tx was similar for the different antibodies of the anti\u2010<jats:italic>\u03b1<\/jats:italic>Gal polymorphism, and was only partially neutralized in vitro with the specific <jats:italic>\u03b1<\/jats:italic>Gal oligosaccharide.<\/jats:p>","DOI":"10.1111\/j.1399-3089.2004.00152.x","type":"journal-article","created":{"date-parts":[[2004,8,10]],"date-time":"2004-08-10T09:28:26Z","timestamp":1092130106000},"page":"408-415","source":"Crossref","is-referenced-by-count":17,"title":["Failure to deplete anti\u2010Gal\u03b11\u20103Gal antibodies after pig\u2010to\u2010baboon organ xenotransplantation by immunoaffinity columns containing multiple Gal\u03b11\u20103Gal oligosaccharides"],"prefix":"10.1111","volume":"11","author":[{"given":"Rafael","family":"Ma\u00f1ez","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nieves","family":"Domenech","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alberto","family":"Centeno","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eduardo","family":"Lopez\u2010Pelaez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabian","family":"Crespo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alberto","family":"Juffe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rudolf O.","family":"Duthaler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas G.","family":"Katopodis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2004,6,29]]},"reference":[{"key":"e_1_2_17_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.3780086"},{"key":"e_1_2_17_3_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1399-3089.2003.02092.x"},{"key":"e_1_2_17_4_2","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-199501000-00024"},{"key":"e_1_2_17_5_2","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-199801270-00005"},{"key":"e_1_2_17_6_2","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-199806270-00006"},{"key":"e_1_2_17_7_2","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-199806270-00008"},{"key":"e_1_2_17_8_2","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-200203270-00009"},{"key":"e_1_2_17_9_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI2134"},{"key":"e_1_2_17_10_2","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-200012270-00002"},{"key":"e_1_2_17_11_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1399-3089.2000.00068.x"},{"key":"e_1_2_17_12_2","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-199809150-00014"},{"key":"e_1_2_17_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-4975(99)00488-9"},{"key":"e_1_2_17_14_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1399-3089.2001.00082.x"},{"key":"e_1_2_17_15_2","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-199609150-00025"},{"key":"e_1_2_17_16_2","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-200112150-00007"},{"key":"e_1_2_17_17_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-60572-7_9"},{"key":"e_1_2_17_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)64004-7"},{"key":"e_1_2_17_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0041-1345(00)01027-7"},{"key":"e_1_2_17_20_2","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-199110000-00024"}],"container-title":["Xenotransplantation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1399-3089.2004.00152.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1399-3089.2004.00152.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,10]],"date-time":"2023-10-10T00:41:43Z","timestamp":1696898503000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1399-3089.2004.00152.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,6,29]]},"references-count":19,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2004,9]]}},"alternative-id":["10.1111\/j.1399-3089.2004.00152.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1399-3089.2004.00152.x","archive":["Portico"],"relation":{},"ISSN":["0908-665X","1399-3089"],"issn-type":[{"value":"0908-665X","type":"print"},{"value":"1399-3089","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,6,29]]}}}