{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,13]],"date-time":"2025-09-13T15:48:28Z","timestamp":1757778508851},"reference-count":15,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2006,10,2]],"date-time":"2006-10-02T00:00:00Z","timestamp":1159747200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Xenotransplantation"],"published-print":{"date-parts":[[2006,11]]},"abstract":"<jats:p><jats:bold>Abstract:\u2002<\/jats:bold><jats:bold> Introduction:\u2002<\/jats:bold> Derangements of coagulation, e.g. thrombotic microangiopathy (TM) and disseminated intravascular coagulation (DIC), limit the success of pig\u2010to\u2010baboon organ transplantation. Studies have investigated the coagulation profile in baboons post\u2010xenotransplantation (XTx), but an extended coagulation profile of healthy baboons pre\u2010XTx has not been reported.<\/jats:p><jats:p><jats:bold>Methods:\u2002<\/jats:bold> Blood was drawn from nine healthy male baboons (approximate age 5\u2003yr, mean weight 15\u2003kg) that had not undergone any prior surgical or therapeutic procedures. An extended coagulation profile, consisting of markers of thrombin activation, fibrinolysis, endothelial activation, the protein C pathway, and overall reactive state, was investigated by a reference coagulation laboratory, using tests for human plasma. The mean value\u2003\u00b1\u2003SD was calculated for 18 parameters (analytes); values outside of the mean\u2003\u00b1\u20032\u2003SD were excluded. Three baboons subsequently underwent transplantation with hearts from GT\u2010KO pigs, and received either no therapy (B24603), CVF (B25003), or CVF\u2003+\u2003leflunomide (B24903), and their extended coagulation parameters were followed.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> For 14 of the 18 analytes, the human reference range reflected the coagulation status of healthy baboons. Exceptions included thrombin\/antithrombin complex and fibrinopeptide A, which were elevated compared with the human reference range, while plasminogen activity was lower. The human assay failed to detect baboon plasminogen activator inhibitor\u20101. Immediately after GT\u2010KO pig heart transplantation, the untreated B24603 demonstrated a coagulation profile consistent with its postoperative clinical status; DIC was not apparent, and the heart was electively excised within 2.5\u2003h. In B25003 and B24903, that rejected their grafts on days 8 and 12, respectively, the coagulation profile showed evidence of DIC, particularly in B24903, which was clinically coagulopathic by this time.<\/jats:p><jats:p><jats:bold>Conclusions:\u2002<\/jats:bold> (i) The human reference range of extended coagulation parameters forms a basis for studies in baboons, with a few exceptions. (ii) Antibody\u2010mediated rejection of GT\u2010KO pig hearts in baboons can be associated with laboratory and clinical evidence of DIC.<\/jats:p>","DOI":"10.1111\/j.1399-3089.2006.00342.x","type":"journal-article","created":{"date-parts":[[2006,10,2]],"date-time":"2006-10-02T08:47:37Z","timestamp":1159778857000},"page":"522-528","source":"Crossref","is-referenced-by-count":24,"title":["Extended coagulation profiles of healthy baboons and of baboons rejecting GT\u2010KO pig heart grafts"],"prefix":"10.1111","volume":"13","author":[{"given":"Mohamed","family":"Ezzelarab","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrea","family":"Cortese\u2010Hassett","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David K. C.","family":"Cooper","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark H.","family":"Yazer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,10,23]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-6143.2004.00353.x"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-3089.2004.00155.x"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm1171"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.tp.0000181397.41143.fa"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.mbc.0000187905.54087.91"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-199812150-00006"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-200011150-00010"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1600-6135.2003.00258.x"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-200206150-00005"},{"key":"e_1_2_6_11_2","first-page":"236","article-title":"Clinical parameters of the normal baboons (Papio species) and chimpanzees (Pan troglodytes)","volume":"43","author":"Hainsey BM","year":"1993","journal-title":"Lab Anim Sci"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0684.1999.tb00085.x"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-3089.2004.00171.x"},{"key":"e_1_2_6_14_2","first-page":"173","volume-title":"Experimental Techniques in Transplantation","author":"Cooper DKC","year":"1994"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/0049-3848(76)90144-4"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V57.6.1140.1140"}],"container-title":["Xenotransplantation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1399-3089.2006.00342.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1399-3089.2006.00342.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,14]],"date-time":"2023-10-14T07:28:59Z","timestamp":1697268539000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1399-3089.2006.00342.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,10,23]]},"references-count":15,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2006,11]]}},"alternative-id":["10.1111\/j.1399-3089.2006.00342.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1399-3089.2006.00342.x","archive":["Portico"],"relation":{},"ISSN":["0908-665X","1399-3089"],"issn-type":[{"value":"0908-665X","type":"print"},{"value":"1399-3089","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,10,23]]}}}