{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,31]],"date-time":"2023-10-31T05:41:16Z","timestamp":1698730876596},"reference-count":19,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2011,8,18]],"date-time":"2011-08-18T00:00:00Z","timestamp":1313625600000},"content-version":"vor","delay-in-days":48,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Xenotransplantation"],"published-print":{"date-parts":[[2011,7]]},"abstract":"<jats:p>Suszynski TM, Rizzari MD, Kidder LS, Mueller K, Chapman CS, Kitzmann JP, Pongratz RL, Cline GW, Todd PW, Kennedy DJ, O\u2019Brien TD, Avgoustiniatos ES, Schuurman H\u2010J, Papas KK. Paramagnetic microparticles do not elicit islet cytotoxicity with co\u2010culture or host immune reactivity after implantation. Xenotransplantation 2011; 18: 239\u2013244. \u00a9 2011 John Wiley &amp; Sons A\/S.<\/jats:p><jats:p><jats:bold>Abstract:\u2002<\/jats:bold><jats:bold> Background:\u2002<\/jats:bold> Paramagnetic microparticles (MPs) may be useful in pancreatic islet purification, in particular purification of porcine islets as a potential xenotransplantation product. We assessed whether MPs affect islet function or induce an adverse effect following implantation.<\/jats:p><jats:p><jats:bold>Methods:\u2002<\/jats:bold> Porcine islets were co\u2010cultured with 0, 500, and 1500 MPs per islet equivalent (IE) for 1\u2003day and with 0 and 1500 MPs\/IE for 7\u2003days. Fractional viability was assessed using oxygen consumption rate normalized to DNA content (OCR\/DNA) and after 7\u2010day co\u2010culture by perifusion glucose\u2010stimulated insulin secretion (GSIS) and by transplantation under the renal capsule of diabetic nude mice. To assess an inflammatory response or immune reaction, MPs (\u223c10<jats:sup>7<\/jats:sup>) were implanted under the renal capsule of C57BL\/6 mice.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> No statistically significant differences were measured in OCR\/DNA (mean\u2003\u00b1\u2003SE) following 1\u2010day co\u2010culture with 0, 500, or 1500 MPs\/IE (243.3\u2003\u00b1\u20034.5, 211.3\u2003\u00b1\u20038.1, or 230.6\u2003\u00b1\u200311.3\u2003nmol\/min\u00b7mgDNA, respectively) or following 7\u2010day co\u2010culture with 0 or 1500 MPs\/IE (248.5\u2003\u00b1\u20031.4 or 252.9\u2003\u00b1\u20034.7\u2003nmol\/min\u00b7mgDNA, respectively). GSIS was not affected by the presence of MPs; first\u2010 and second\u2010phase insulin area\u2010under\u2010the\u2010curve (mean\u2003\u00b1\u2003SE) reflected no statistically significant differences after 7\u2010day co\u2010culture between 0 and 1500 MPs\/IE (8.36\u2003\u00b1\u20030.29 and 8.45\u2003\u00b1\u20030.70\u2003pg\/ml\u00b7min\u00b7ngDNA for first\u2010phase; 69.73\u2003\u00b1\u20032.18 and 65.70\u2003\u00b1\u20034.34\u2003pg\/ml\u00b7min\u00b7ngDNA for second\u2010phase, respectively). Islets co\u2010cultured with MPs normalized hyperglycemia in diabetic nude mice, suggesting no adverse effects on in vivo islet function. Implantation of MPs did not elicit tissue injury, inflammatory change or immune reactivity.<\/jats:p><jats:p><jats:bold>Conclusion:\u2002<\/jats:bold> MPs do not adversely affect islet viability or function during co\u2010culture, and MPs are not immune reactive following implantation.<\/jats:p>","DOI":"10.1111\/j.1399-3089.2011.00648.x","type":"journal-article","created":{"date-parts":[[2011,8,18]],"date-time":"2011-08-18T06:28:23Z","timestamp":1313648903000},"page":"239-244","source":"Crossref","is-referenced-by-count":3,"title":["Paramagnetic microparticles do not elicit islet cytotoxicity with co\u2010culture or host immune reactivity after implantation"],"prefix":"10.1111","volume":"18","author":[{"given":"Thomas M.","family":"Suszynski","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael D.","family":"Rizzari","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Louis 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J.","family":"Kennedy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Timothy D.","family":"O\u2019Brien","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Efstathios S.","family":"Avgoustiniatos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Henk\u2010Jan","family":"Schuurman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Klearchos 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