{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T18:14:49Z","timestamp":1791137689237,"version":"4.1.0"},"reference-count":35,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2006,3,15]],"date-time":"2006-03-15T00:00:00Z","timestamp":1142380800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vox Sanguinis"],"published-print":{"date-parts":[[2006,5]]},"abstract":"<jats:p><jats:bold>Background and Objectives\u2002<\/jats:bold> Three recent probable cases of transmission of a variant of human Creutzfeldt\u2013Jakob disease (vCJD) through blood transfusion suggest that the disease can be transmitted through transfusion of blood components from presymptomatic blood donors. In this study, we investigated the performance of a new filter for reducing the levels of infectious prions (PrP<jats:sup>Sc<\/jats:sup>) from red cell concentrates (RCC).<\/jats:p><jats:p><jats:bold>Materials and Methods\u2002<\/jats:bold>  <jats:italic>Endogenous Infectivity:<\/jats:italic> A pool of 500\u00a0ml of whole blood was collected from 263K\u2010strain scrapie\u2010infected hamsters into an anticoagulant, processed into non\u2010leucoreduced RCC (NL\u2010RCC), and then passed through a prion\u2010reduction filter. Pre\u2010 and postfiltration samples were tested for PrP<jats:sup>Sc<\/jats:sup> by Western blot and infectivity by inoculation of healthy hamsters. Results of the endogenous infectivity study after 200\u00a0days post\u2010inoculation are discussed. <jats:italic>Exogenous (Spiking) Study:<\/jats:italic> Scrapie\u2010infected hamster brain homogenates containing PrP<jats:sup>Sc<\/jats:sup> were added to human RCC and then filtered. Levels of PrP<jats:sup>Sc<\/jats:sup> were determined by Western blot assay. The effect of prior leucodepletion of \u2018spiked\u2019 RCC on PrP<jats:sup>Sc<\/jats:sup> removal by the prion\u2010removal filter was also assessed.<\/jats:p><jats:p><jats:bold>Results<\/jats:bold> In the endogenous infectivity study, at 200\u2010day observation time, the prefiltered RCC transmitted disease to six of the 187 hamsters, whereas the filtered RCC did not transmit disease to any of 413 animals, <jats:italic>P<\/jats:italic>\u00a0=\u00a00\u00b7001. The prion filter also significantly reduced the concentration of leucocytes in the RCC by about 4 logs, <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00\u00b705. In the exogenous (spiking) study, the level of PrP<jats:sup>res<\/jats:sup> was significantly reduced in RCC <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00\u00b705. Prior leucodepletion of the RCC with a leucoreduction filter did not significantly reduce the concentration of exogenously spiked PrP<jats:sup>Sc<\/jats:sup>, <jats:italic>P<\/jats:italic>\u00a0&gt;\u00a00\u00b705.<\/jats:p><jats:p><jats:bold>Conclusion\u2002<\/jats:bold> The use of this new prion\u2010reduction filter should reduce the risk of vCJD transmission through transfusion of RCC, the most widely transfused blood component.<\/jats:p>","DOI":"10.1111\/j.1423-0410.2006.00765.x","type":"journal-article","created":{"date-parts":[[2006,3,15]],"date-time":"2006-03-15T14:15:30Z","timestamp":1142432130000},"page":"265-275","source":"Crossref","is-referenced-by-count":28,"title":["Pall leukotrap affinity prion\u2010reduction filter removes exogenous infectious prions and endogenous infectivity from red cell concentrates"],"prefix":"10.1111","volume":"90","author":[{"given":"S. O.","family":"Sowemimo\u2010Coker","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Pesci","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Andrade","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. B.","family":"Kascsak","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. 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University of Edinburgh UK.http:\/\/www.cjd.ed.ac.uk\/"},{"key":"e_1_2_5_9_2","unstructured":"Australian Government\u2010Department of Health and Ageing:What is Variant Creutzfeldt\u2013Jakob Disease(updated December 2004).http:\/\/www.health.gov.au\/internet\/wcms\/Publishing.nsf\/Content\/health\u2010pubhlth\u2010strateg\u2010bse\u2010faq.htm"},{"key":"e_1_2_5_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(00)02719-7"},{"key":"e_1_2_5_11_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-83-11-2897"},{"key":"e_1_2_5_12_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.1998.38998408999.x"},{"key":"e_1_2_5_13_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.0041-1132.2003.00586.x"},{"issue":"39","key":"e_1_2_5_14_2","first-page":"1169","article-title":"Further studies of blood infectivity in experimental model of transmissible spongiform encephalopathy, with an explanation of why blood components do not transmit Creutzfeldt\u2013Jakob disease in humans","volume":"1999","author":"Brown P","journal-title":"Transfusion"},{"key":"e_1_2_5_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(04)15486-X"},{"key":"e_1_2_5_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(04)16811-6"},{"key":"e_1_2_5_17_2","unstructured":"United Kingdom Health Protection Agency (HPA): New case of variant CJD associated with blood transfusion (updated 9 February 2006).http:\/\/www.hpa.org.uk\/infections\/topics_az\/cjd\/menu.htm"},{"key":"e_1_2_5_18_2","unstructured":"United Kingdom Spongiform Encephalopathy Advisory Committee (SEAC):Annual Report 1997\u20131998. pp10. 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