{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T02:48:18Z","timestamp":1772160498759,"version":"3.50.1"},"reference-count":45,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2006,5,11]],"date-time":"2006-05-11T00:00:00Z","timestamp":1147305600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vox Sanguinis"],"published-print":{"date-parts":[[2006,7]]},"abstract":"<jats:p>\n                    <jats:bold>Background and Objectives\u2002<\/jats:bold>\n                    Single\u2010donor or small\u2010pool cryoprecipitates are produced by blood establishments, mostly in developing countries, for substitute therapy in haemophilia A, von Willebrand disease and fibrinogen deficiency, as well as for the manufacture of fibrin sealant. As cryoprecipitate may be contaminated with pathogenic plasma\u2010borne viruses, there is an urgent need to develop a simple method for the viral inactivation of cryoprecipitate.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Materials and Methods\u2002<\/jats:bold>\n                    Cryoprecipitate was obtained according to standard procedures. Ten minipools of five or six donations of cryoprecipitate were prepared and subjected, in sterile closed bags, to a viral inactivation treatment using either 2% tri(n\u2010)butyl phosphate (TnBP) for 4\u00a0h at 37\u00a0\u00b0C or the combination of 1% TnBP and 1% Triton X\u201045 for 4\u00a0h at 31\u00a0\u00b0C. The cryoprecipitates were subsequently extracted three times in their processing bags by mixing and decantation using 7\u00b75% sterile ricinus oil. The TnBP\u2010treated cryoprecipitates were further subjected to a clarifying centrifugation step at 3800\u00a0\n                    <jats:bold>\n                      <jats:italic>g<\/jats:italic>\n                    <\/jats:bold>\n                    for 30\u00a0min. The final products were dispensed into individual bags and frozen at \u221230\u00a0\u00b0C or lower.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Results\u2002\u2002<\/jats:bold>\n                    The cryoprecipitates treated with either 2% TnBP or 1% TnBP\u00a0+\u00a01% Triton X\u201045 showed excellent (&gt;\u00a093%) mean recovery of coagulant factor VIII (FVIII), ristocetin cofactor Von Willebrand factor (VWF:RCo), and clottable fibrinogen activity. Prothrombin time, international normalized ratio and activated partial thromboplastin time increased during solvent\u2013detergent treatment but returned to initial values after oil extractions. The final content of TnBP and Triton X\u201045 was &lt;\u00a010 and 50\u00a0ppm, indicating excellent removal by the oil\u2010extraction procedure.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Conclusions\u2002<\/jats:bold>\n                    Viral inactivation treatment by TnBP, with or without Triton X\u201045, can be applied to minipools of cryoprecipitate, with good recovery of FVIII, VWF and fibrinogen. The viral inactivation and solvent\u2013detergent removal process can be performed in a closed bag system and using simple blood establishment techniques and equipment. This technology could be considered for the improved viral safety of cryoprecipitate which is used to treat haemophilia A, von Willebrand disease or fibrinogen deficiency, or to prepare fibrin sealant.\n                  <\/jats:p>","DOI":"10.1111\/j.1423-0410.2006.00772.x","type":"journal-article","created":{"date-parts":[[2006,5,11]],"date-time":"2006-05-11T11:56:31Z","timestamp":1147348591000},"page":"56-62","source":"Crossref","is-referenced-by-count":29,"title":["A minipool process for solvent\u2013detergent treatment of cryoprecipitate at blood centres using a disposable bag system"],"prefix":"10.1111","volume":"91","author":[{"given":"T.","family":"Burnouf","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. A.","family":"Goubran","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Radosevich","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. A.","family":"Sayed","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.","family":"Gorgy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"El\u2010Ekiaby","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,5,11]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2516.2004.01007.x"},{"key":"e_1_2_6_3_2","first-page":"1","volume-title":"World Federation of Hemophilia Guide to Developing a National Patient Registry.","author":"Evatt B","year":"2005"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2516.1999.00317.x"},{"key":"e_1_2_6_5_2","first-page":"1","volume-title":"The Preparation of Single Donor Cryoprecipitate \u2013 Revised Edition.","author":"Lloyd S","year":"2004"},{"key":"e_1_2_6_6_2","first-page":"S293","article-title":"Lyophilized cryoprecipitate for children with hemophilia A","volume":"85","author":"Nuchprayoon I","year":"2002","journal-title":"J Med Assoc Thai"},{"key":"e_1_2_6_7_2","first-page":"188","article-title":"Haemostasis with cryoprecipitate in patients undergoing surgery for severe von Willebrand disease","volume":"13","author":"Mathews V","year":"2000","journal-title":"Natl Med J India"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2516.1999.00337.x"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1055\/s-2005-922221"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1055\/s-2005-922231"},{"key":"e_1_2_6_11_2","first-page":"1","volume-title":"Local Hemostatic Blood Products: Fibrin Sealant and Platelet Gel. Monograph.","author":"Burnouf T","year":"2004"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1309\/56MQVQAQG8YU90X9"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2005.04.001"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1590\/S0074-02762005000100002"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268803008653"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1080\/00365540210147804"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1997.57.66"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0035-9203(97)90070-5"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(04)16587-2"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.20001"},{"key":"e_1_2_6_21_2","first-page":"S69","article-title":"The efficacy and safety of lyophilized cryoprecipitate in hemophilia A","volume":"82","author":"Chuansumrit A","year":"1999","journal-title":"J Med Assoc Thai"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.1985.25686071422.x"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V79.3.826.826"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1054\/blre.2000.0129"},{"key":"e_1_2_6_25_2","first-page":"1","article-title":"WHO Expert Committee on Biological Standardization","volume":"924","author":"Horowitz B","year":"2004","journal-title":"World Health Organ Tech Rep Ser"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1423-0410.2003.00265.x"},{"key":"e_1_2_6_27_2","article-title":"Mini\u2010pool solvent\/detergent treatment of plasma for transfusion using a single\u2010use bag system","author":"Burnouf T","journal-title":"Transfusion"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1159\/000205234"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1423-0410.1992.tb01230.x"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1423-0410.1991.tb00864.x"},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1423-0410.1984.tb00045.x"},{"key":"e_1_2_6_32_2","first-page":"4592s","article-title":"Quantitative assays for evaluation of HTLV\u2010III inactivation procedures: tri (N\u2010butyl) phosphate: sodium cholate and beta\u2010propiolactone","volume":"45","author":"Prince AM","year":"1985","journal-title":"Cancer Res"},{"key":"e_1_2_6_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(86)91101-3"},{"key":"e_1_2_6_34_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1423-0410.1987.tb02989.x"},{"key":"e_1_2_6_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/0049-3848(89)90395-2"},{"key":"e_1_2_6_36_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.1990.30790385516.x"},{"key":"e_1_2_6_37_2","unstructured":"WoodsKR OrmeT:New York Blood Center assignee. Removal of lipid\u2010soluble process chemicals from biological materials by extraction with naturally occurring oils or synthetic substitutes thereof.USA patent 4 789 545. 31 March1986."},{"key":"e_1_2_6_38_2","doi-asserted-by":"publisher","DOI":"10.1097\/00001721-199412003-00006"},{"key":"e_1_2_6_39_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00320015"},{"key":"e_1_2_6_40_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01963879"},{"key":"e_1_2_6_41_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1423-0410.1991.tb00907.x"},{"key":"e_1_2_6_42_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2141.1997.d01-1993.x"},{"key":"e_1_2_6_43_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2141.2000.02078.x"},{"key":"e_1_2_6_44_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1423-0410.2000.7930156.x"},{"key":"e_1_2_6_45_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.0958-7578.2004.00528.x"},{"key":"e_1_2_6_46_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2516.2004.00910.x"}],"container-title":["Vox Sanguinis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1423-0410.2006.00772.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1423-0410.2006.00772.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,10]],"date-time":"2023-10-10T06:56:35Z","timestamp":1696920995000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1423-0410.2006.00772.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,5,11]]},"references-count":45,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2006,7]]}},"alternative-id":["10.1111\/j.1423-0410.2006.00772.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1423-0410.2006.00772.x","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.14427.471930","asserted-by":"object"}]},"ISSN":["0042-9007","1423-0410"],"issn-type":[{"value":"0042-9007","type":"print"},{"value":"1423-0410","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,5,11]]}}}