{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,18]],"date-time":"2023-10-18T07:55:58Z","timestamp":1697615758010},"reference-count":23,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2006,6,29]],"date-time":"2006-06-29T00:00:00Z","timestamp":1151539200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vox Sanguinis"],"published-print":{"date-parts":[[2006,10]]},"abstract":"<jats:p><jats:bold>Background and Objectives\u2002<\/jats:bold> Positive samples identified during routine serological screening for HCV (hepatitis C virus), HBV (hepatitis B virus) and HIV (human immunodeficiency virus) are confirmed by nucleic acid testing in the SNBTS (Scottish National Blood Transfusion Service) PCR Reference laboratory. Serological screening for HTLV\u2010I (human T\u2010cell lymphotropic virus type I) and \u2010II was implemented in Scotland in November 2002, at which time a PCR assay was not available for confirmation. Our aim was to develop a real\u2010time PCR assay that could be used for the confirmation of samples showing HTLV\u2010I serological positive or indeterminate reactivity and to investigate whether a serologically silent carrier status exists (\u2018Tax\u2019 only) in the Scottish donor population.<\/jats:p><jats:p><jats:bold>Materials and Methods\u2002<\/jats:bold> A real\u2010time HTLV PCR was devised using a lymphoblastoid cell line which has HTLV\u2010I sequence integrated in the genome (C8166 cells). These were spiked into peripheral blood mononuclear cells. The assay was evaluated on archived serologically confirmed HTLV\u2010positive samples and new positives identified since implementation of screening.<\/jats:p><jats:p><jats:bold>Results\u2002<\/jats:bold> HTLV\u2010I and \u2010II were detected in cells and plasma from stored donations and a serological positive donation identified in routine screening. HTLV DNA can also be amplified from the plasma obtained from plasma preparation tubes. There was no evidence of a carrier status (\u2018Tax\u2019 only) in 100 serologically negative blood donors tested. The PCR assay developed is reliable and sensitive, capable of identifying one copy of HTLV\u2010I.<\/jats:p><jats:p><jats:bold>Conclusions\u2002<\/jats:bold> The HTLV PCR is a useful addition for HTLV confirmation, especially in serologically indeterminate samples and for look\u2010back studies. HTLV PCR confirmation will provide additional useful information for donor medical staff for counselling donors.<\/jats:p>","DOI":"10.1111\/j.1423-0410.2006.00816.x","type":"journal-article","created":{"date-parts":[[2006,6,30]],"date-time":"2006-06-30T00:14:28Z","timestamp":1151626468000},"page":"231-236","source":"Crossref","is-referenced-by-count":10,"title":["Detection of HTLV\u2010I and \u2010II in Scottish blood donor samples and archive donations"],"prefix":"10.1111","volume":"91","author":[{"given":"F.","family":"Davidson","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Lycett","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L. 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