{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T12:12:41Z","timestamp":1780488761077,"version":"3.54.1"},"reference-count":32,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2004,7,16]],"date-time":"2004-07-16T00:00:00Z","timestamp":1089936000000},"content-version":"vor","delay-in-days":2633,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["European Journal of Biochemistry"],"published-print":{"date-parts":[[1997,5]]},"abstract":"<jats:p>The inhibition mechanism of the dimeric human placenta glutathione transferase (GST) P1\u20101 by calvatic acid and the reaction intermediates, i.e. the diazocyanide analogue of calvatic acid, has been investigated at pH 7.0 and 30.0\u00b0C. Experiments performed at different molar ratios of inhibitor\/GST P1\u20101 indicate that 1 mol calvatic acid inactivates 1 mol GST PI\u20101, containing two catalytically equivalent active sites. However, 2 rnol of the diazocyanide analogue of calvatic acid inactivate 1 mol GST PI\u20101. Two disulfide bridgesldimer, probably between Cys47 and CyslOl, have been formed during the reaction of GST P1\u20101 with calvatic acid and its diazocyanide analogue. The apparent second\u2010order rate constants for GST P1\u20131 inactivation by calvatic acid and its diazocyanide analogue are 2.4 \u00b1 10.3 M<jats:sup>\u22121<\/jats:sup>s<jats:sup>\u22121<\/jats:sup>and (8.5\u00b10.7)\u00d710<jats:sup>3<\/jats:sup>M<jats:sup>\u22121<\/jats:sup>s<jats:sup>\u22121<\/jats:sup>, respectively. The reaction of calvatic acid with free L\u2010cysteine can be described by a simple process with an apparent second\u2010order rate constant of (5.0\u00b10.4)\u00d710<jats:sup>1<\/jats:sup>M<jats:sup>\u22121<\/jats:sup>s<jats:sup>\u22121<\/jats:sup>. In contrast, a transient species occurs during the reaction of the diazocyanide analogue of calvatic acid with free L\u2010cysteine. Kinetics may be described by a second\u2010order process [the rate constant being (8.0\u00b10.5)\u00d710<jats:sup>3<\/jats:sup>M<jats:sup>\u22121<\/jats:sup>s<jats:sup>\u22121<\/jats:sup>followed by a first\u2010order decay [the rate constant corresponding to (1.2\u00b10.1)\u00d710<jats:sup>\u22121<\/jats:sup>s<jats:sup>\u22121<\/jats:sup>]. Calvatic acid represents an enzyme inhibitor acting much slower than its reaction intermediates (i.e. its diazocyanide analogue).<\/jats:p>","DOI":"10.1111\/j.1432-1033.1997.00663.x","type":"journal-article","created":{"date-parts":[[2004,7,16]],"date-time":"2004-07-16T13:35:48Z","timestamp":1089984948000},"page":"663-667","source":"Crossref","is-referenced-by-count":8,"title":["Inhibition of Human Placenta Glutathione Transferase Pl\u20101 by the Antibiotic Calvatic Acid and its Diazocyanide Analogue"],"prefix":"10.1111","volume":"245","author":[{"given":"Giovanni","family":"Antonini","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giuseppina","family":"Pitari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna Maria","family":"Caccuri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giorgio","family":"Ricci","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donatella","family":"Boschi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberta","family":"Fruttero","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alberto","family":"Gasco","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paolo","family":"Ascenzi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2004,7,16]]},"reference":[{"key":"e_1_2_3_2_1","doi-asserted-by":"crossref","first-page":"4676","DOI":"10.1016\/S0021-9258(18)32471-2","article-title":"Transient removal of proflavine inhibition of bovine p\u2010trypsin by the bovine basic pancreatic trypsin inhibitor (Kunitz): a case for \u2018chronosteric effects\u2019","volume":"258","author":"Antonini E.","year":"1983","journal-title":"J. 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