{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T12:32:51Z","timestamp":1791289971637,"version":"4.3.0"},"reference-count":54,"publisher":"Wiley","issue":"1-2","license":[{"start":{"date-parts":[[2004,7,16]],"date-time":"2004-07-16T00:00:00Z","timestamp":1089936000000},"content-version":"vor","delay-in-days":2753,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["European Journal of Biochemistry"],"published-print":{"date-parts":[[1997,1]]},"abstract":"<jats:p>\n                    Axonin\u20101, a member of the immunoglobulin\/fibronectin type\u2010III family of cell\u2010adhesion molecules, occurs both as a glycosylphosphatidylinositol\u2010(glycosylPtdIns)\u2010anchored membrane\u2010bound and a soluble form. In vivo observations show that the major part of axonin\u20101 is found in the soluble fraction and that soluble axonin\u20101 perturbs neurite fasciculation and pathfinding in the developing chicken embryo. This has prompted further investigations into the mechanism of the axonin\u20101 release. We demonstrate here that axonin\u20101 released from dorsal root ganglion neurons contains ethanolamine and inositol, components of the glycosylPtdIns anchor. Secreted axonin\u20101 does not exhibit the cross\u2010reacting determinant epitope, an indication that the cleavage of the anchor is not mediated by a phosphatidylinositol\u2010specific phospholipase C. Treatment of dorsal root ganglion neurons with 1,10\u2010phenanthroline, an inhibitor of glycosylPtd\u2010Ins\u2010specific phospholipase D, reduces the release of axonin\u20101 by 56%. Moreover, glycosylPtdIns\u2010specific phospholipase D activity was detected in dorsal root ganglion neurons and brain. These results suggest that axonin\u20101 is released from the membrane by an endogenously expressed glycosylPtdIns\u2010specific phospholipase D\n                    <jats:italic>in vivo<\/jats:italic>\n                    With domain\u2010swaping experiments between axonin\u20101 and its non\u2010released relative F11, deletion mutants and monoclonal antibodies, we demonstrate that the fourth fibronectin type\u2010III\u2010like domain of axonin\u20101 is required for the generation of the soluble form of axonin\u20101.\n                  <\/jats:p>","DOI":"10.1111\/j.1432-1033.1997.0502a.x","type":"journal-article","created":{"date-parts":[[2004,7,16]],"date-time":"2004-07-16T10:33:00Z","timestamp":1089973980000},"page":"502-510","source":"Crossref","is-referenced-by-count":30,"title":["The Neuronal Cell\u2010Adhesion Molecule Axonin\u20101 is Specifically Released by an Endogenous Glycosylphosphatidylinositol\u2010Specific Phospholipase"],"prefix":"10.1111","volume":"243","author":[{"given":"Ruth","family":"Lierheimer","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beat","family":"Kunz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lorenz","family":"Vogt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reto","family":"Savoca","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Urs","family":"Brodbeck","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Sonderegger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2004,7,16]]},"reference":[{"key":"e_1_2_2_2_1","doi-asserted-by":"crossref","first-page":"661","DOI":"10.1042\/bj2980661","article-title":"Saponin induced release of cell\u2010surface\u2010anchored\u2010Thy\u20101 by serum glycosylphosphatidylinositol\u2010specific phospholipase D","volume":"298","author":"Bergman A. 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