{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,25]],"date-time":"2025-10-25T14:08:19Z","timestamp":1761401299512},"reference-count":20,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2006,6,16]],"date-time":"2006-06-16T00:00:00Z","timestamp":1150416000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Anat Histol Embryol"],"published-print":{"date-parts":[[2006,12]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Rolling Mouse Nagoya (RMN) carries a mutation in a gene encoding for <jats:italic>\u03b1<\/jats:italic><jats:sub>1A<\/jats:sub> subunit of P\/Q\u2010type Ca<jats:sup>2+<\/jats:sup> channel (Ca<jats:sub>v<\/jats:sub>2.1). In addition to ataxia, this mutant mouse exhibits abnormal hindlimb extension, which is characterized by a sustained excessive tone of hindlimb extensor muscles. This study aimed to clarify whether serotonergic (5\u2010HTergic) innervation of the spinal motoneurons was altered in RMN in relation to the abnormal hindlimb extension. The density of 5\u2010HT immunoreactive fibres in the ventral horn of lumbar and sacral regions of spinal cord was significantly greater in RMN than in controls. Retrograde wheat germ agglutinin\u2010conjugated horseradish peroxidase (WGA\u2010HRP) labelling combined with 5\u2010HT immunostaining revealed that the number of 5\u2010HT immunoreactive terminals adjoining femoris quadriceps motoneurons was about 2.5\u2010fold greater in RMN than in controls. Furthermore, 5\u2010HT immunostaining in the lumbar cord ventral horn was examined in three other Ca<jats:sub>v<\/jats:sub>2.1 mutant mice (<jats:italic>tottering<\/jats:italic>, <jats:italic>leaner<\/jats:italic> and <jats:italic>pogo<\/jats:italic>) as to whether or not they showed the abnormal hindlimb extension. Among these mutants, the increased density of 5\u2010HT immunoreactive fibres was observed in correlation with the presence of the abnormal hindlimb extension. The results suggest an increased 5\u2010HTergic innervation of the lumbosacral motoneurons in correlation with the abnormal hindlimb extension in RMN and other Ca<jats:sub>v<\/jats:sub>2.1 mutant mice. As 5\u2010HT is known to induce the sustained membrane depolarizations without continuous excitatory synaptic inputs (plateau potentials) in spinal motoneurons, the increased 5\u2010HTergic innervation may cause the sustained excitation of hindlimb extensor motoneurons, resulting in the abnormal hindlimb extension.<\/jats:p>","DOI":"10.1111\/j.1439-0264.2006.00697.x","type":"journal-article","created":{"date-parts":[[2006,6,16]],"date-time":"2006-06-16T15:05:55Z","timestamp":1150470355000},"page":"387-392","source":"Crossref","is-referenced-by-count":5,"title":["Increased Serotonergic Innervation of Lumbosacral Motoneurons of Rolling Mouse Nagoya in Correlation with Abnormal Hindlimb Extension"],"prefix":"10.1111","volume":"35","author":[{"given":"Y.","family":"Koyanagi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Sawada","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Sakata\u2010Haga","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y.\u2010G.","family":"Jeong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y.","family":"Fukui","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,9,25]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9861(19980330)393:1<69::AID-CNE7>3.0.CO;2-O"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1997.77.3.1362"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3940(98)00959-8"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1124\/mol.55.1.23"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9861(19980727)397:2<251::AID-CNE7>3.0.CO;2-#"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-9525(97)01370-X"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81381-1"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.jhered.a107180"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-3806(00)00114-0"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0959-4388(98)80117-7"},{"key":"e_1_2_6_12_1","doi-asserted-by":"crossref","first-page":"5654","DOI":"10.1523\/JNEUROSCI.20-15-05654.2000","article-title":"Reduced voltage sensitivity of activation of P\/Q\u2010type Ca2+ channels is associated with the ataxic mouse mutation rolling Nagoya (tg\n                  \n                     rol\n                  \n                  )","volume":"20","author":"Mori Y.","year":"2000","journal-title":"J. Neurosci."},{"key":"e_1_2_6_13_1","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1538\/expanim1957.22.4_281","article-title":"The observation of rolling mouse Nagoya (rol), a new neurological mutant, and its maintenance","volume":"22","author":"Oda S.","year":"1973","journal-title":"Exp. 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