{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,21]],"date-time":"2023-11-21T11:45:55Z","timestamp":1700567155136},"reference-count":23,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2012,1,20]],"date-time":"2012-01-20T00:00:00Z","timestamp":1327017600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Anat Histol Embryol"],"published-print":{"date-parts":[[2012,8]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>The aim of this study was to use culture medium (<jats:styled-content style=\"fixed-case\">M<\/jats:styled-content>c<jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>oy\u00ae) as a test to evaluate the classification of embryos after a primary grading using stereoscopic microscopy to further confirm whether embryos have been correctly scored by stereoscopic microscopy evaluating the level of apoptosis. Forty\u2010six <jats:italic><jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>os indicus<\/jats:italic> embryos were collected with a non\u2010surgical method and evaluated with stereoscopic microscopy for scoring in three categories (good, fair and poor). Cell proliferation and apoptosis were assessed and compared between the control group (<jats:italic>n<\/jats:italic>\u00a0=\u00a014) at the onset of the experiment and in an experimental group (<jats:italic>n<\/jats:italic>\u00a0=\u00a032) after stored for 4\u00a0h in a culture medium. Embryos were processed using <jats:styled-content style=\"fixed-case\">TUNEL<\/jats:styled-content> and BrdU markers to determine the apoptosis and cell proliferation. Seventy\u2010four per cent of good quality embryos developed favourably after the 4\u00a0h of culture; 60% of fair embryos maintained their evolution, while 100% of poor quality embryos presented degenerative changes from the beginning. No statistical differences were found between the experimental and the control groups in the count of positive BrdU and apoptotic nuclei. In poor quality embryos, a higher number of apoptotic cells were found relative to good and fair embryos, both in the experimental and control groups (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05). These results suggest that the culture medium may be used for a short time as a fast, practical and non\u2010invasive alternative to further confirm whether embryos have been correctly scored by stereoscopic microscopy.<\/jats:p>","DOI":"10.1111\/j.1439-0264.2011.01133.x","type":"journal-article","created":{"date-parts":[[2012,1,20]],"date-time":"2012-01-20T11:27:19Z","timestamp":1327058839000},"page":"274-279","source":"Crossref","is-referenced-by-count":2,"title":["Use of a Culture Medium (<scp>M<\/scp>c<scp>C<\/scp>oy\u00ae), as a Method for Evaluating <i><scp>B<\/scp>os indicus\u00a0<\/i>\u00d7\u00a0<i><scp>B<\/scp>os taurus<\/i> Embryos"],"prefix":"10.1111","volume":"41","author":[{"given":"B.","family":"Godinez","sequence":"first","affiliation":[{"name":"Departamento de Reproducci\u00f3n Animal, Facultad de Medicina Veterinaria y Zootecnia Universidad Nacional Aut\u00f3noma de M\u00e9xico, Mexico City, Mexico PC 04510"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C. S.","family":"Galina","sequence":"additional","affiliation":[{"name":"Departamento de Reproducci\u00f3n Animal, Facultad de Medicina Veterinaria y Zootecnia Universidad Nacional Aut\u00f3noma de M\u00e9xico, Mexico City, Mexico PC 04510"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N.","family":"Moreno\u2010Mendoza","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda Celular y Fisiolog\u00eda, Instituto de Investigaciones Biom\u00e9dicas Universidad Nacional Aut\u00f3noma de M\u00e9xico, Tuxpan, Veracruz, Mexico CP 92800"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Alarcon","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias Biol\u00f3gicas y Agropecuarias Universidad Veracruzana, Tuxpan, Veracruz, Mexico CP 92800"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. A.","family":"Lammoglia","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias Biol\u00f3gicas y Agropecuarias Universidad Veracruzana, Tuxpan, Veracruz, Mexico CP 92800"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,1,20]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1439-0531.2002.t01-1-00379.x"},{"key":"e_1_2_6_3_1","first-page":"42","article-title":"Desenvolvimento in vitro de embri\u00f5es bovinos de m\u00e1 qualidade submetidos a tr\u00eas diferentes meios de cultura","volume":"32","author":"Alvarez R. H.","year":"2004","journal-title":"Acta Scientiae Veterinariae"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2005.07.012"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2007.05.015"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0967199402002253"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.S0022-0302(89)79081-0"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0093-691X(95)00189-F"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(02)01243-8"},{"key":"e_1_2_6_10_1","volume-title":"Biolog\u00eda del Desarrollo","author":"Gilbert S. F.","year":"2005"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.1010257"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.S0022-0302(92)78049-7"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0093-691X(83)90201-7"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod66.2.386"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod65.1.52"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0531.2004.00492.x"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(02)01246-3"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(88)80053-0"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/0093-691X(89)90022-8"},{"key":"e_1_2_6_20_1","first-page":"103","volume-title":"Manual of the International Embryo Transfer Society","author":"Robertson I.","year":"1998"},{"key":"e_1_2_6_21_1","first-page":"526","article-title":"In vivo culture of In vivo\u2010derived grade 3 bovine embryos increases development and quality","volume":"57","author":"Romo S.","year":"2002","journal-title":"Theriogenology"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/0093-691X(95)00190-J"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/12.7.1531"},{"key":"e_1_2_6_24_1","first-page":"29","article-title":"Development and application of ovum pick up (OPU) and in vitro embryo production in the bovine. A view to arTech experiences","author":"Vivanco\u2010Mackie H.","year":"2000","journal-title":"Proc. Australian Embryo Transfer Society, Perth Conference. \u201cET Beyond 2000\u201d, Observation City, WA. Australia. 28\u201330 June, 2000. pp."}],"container-title":["Anatomia, Histologia, Embryologia"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1439-0264.2011.01133.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1439-0264.2011.01133.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,21]],"date-time":"2023-11-21T11:22:12Z","timestamp":1700565732000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1439-0264.2011.01133.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,1,20]]},"references-count":23,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2012,8]]}},"alternative-id":["10.1111\/j.1439-0264.2011.01133.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1439-0264.2011.01133.x","archive":["Portico"],"relation":{},"ISSN":["0340-2096","1439-0264"],"issn-type":[{"value":"0340-2096","type":"print"},{"value":"1439-0264","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,1,20]]}}}