{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T18:07:48Z","timestamp":1773425268844,"version":"3.50.1"},"reference-count":23,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2007,6,5]],"date-time":"2007-06-05T00:00:00Z","timestamp":1181001600000},"content-version":"vor","delay-in-days":4,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Animal Breeding Genetics"],"published-print":{"date-parts":[[2007,6]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Insulin\u2010like growth factor\u2010binding protein acid\u2010labile subunit (IGFALS) is an important glycoprotein in the circulation complex for insulin\u2010like growth factors\u2010I and \u2010II (IGFs). So far only a partial porcine <jats:italic>IGFALS<\/jats:italic> sequence is available. We herein report the isolation, characterization, polymorphism identification and expression analysis of the porcine <jats:italic>IGFALS<\/jats:italic> gene. A 2000\u2010bp full\u2010length cDNA sequence was determined with 5\u2032\u2010 and 3\u2032\u2010Rapid Amplification of cDNA End (RACE) assay. It contains a 1821\u2010bp open reading frame encoding a protein of 606 amino acids with a calculated molecular mass of 66\u2003kDa and a theoretical isoelectric point of 6.89. The deduced IGFALS protein shares high identity (70\u201380%) with other mammalian IGFALS. We also obtained a full\u2010length genomic DNA sequence of the gene. Similar to the other mammalian orthologues, particularly in terms of exon size and exon\/intron boundaries, the porcine <jats:italic>IGFALS<\/jats:italic> gene spans a transcription unit of 2990\u2003bp, consisting of two exons and one intron. Three single nucleotide polymorphisms (synonymous mutations) were identified in the DNA sequence. RT\u2010PCR assays indicate that <jats:italic>IGFALS<\/jats:italic> is expressed ubiquitously in pigs and transcripts are particularly abundant in the liver, lung, white adipose tissue, prostate, epididymis, thyroid and bladder.<\/jats:p>","DOI":"10.1111\/j.1439-0388.2007.00645.x","type":"journal-article","created":{"date-parts":[[2007,6,5]],"date-time":"2007-06-05T10:24:33Z","timestamp":1181039073000},"page":"133-138","source":"Crossref","is-referenced-by-count":6,"title":["Characterization of the porcine <i>insulin\u2010like growth factor\u2010binding protein, acid\u2010labile subunit<\/i> gene: full\u2010length cDNA and DNA sequence, polymorphisms and expression profile"],"prefix":"10.1111","volume":"124","author":[{"given":"S.","family":"Li","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Ren","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Huang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,6,5]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1210\/jcem-70-5-1347"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1159\/000184186"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.18.6898"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.19.10028"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.134.6.2498"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2002-020308"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.135.6.2335"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1996.1602"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1677\/jme.0.0190267"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa013100"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1530\/acta.0.1210753"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.33.23328"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.82.12.3944"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1677\/jme.0.0260135"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2052.2005.01314.x"},{"key":"e_1_2_6_17_1","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1007\/978-1-59259-712-3_6","volume-title":"Contemporary Endocrinology: the IGF System","author":"Ooi G.T.","year":"1999"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0083-6729(08)60444-6"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.141.4.1425"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.141.2.833"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.11.6074"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.120172697"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo.141.7.7563"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI117636"}],"container-title":["Journal of Animal Breeding and Genetics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1439-0388.2007.00645.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1439-0388.2007.00645.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,10]],"date-time":"2023-10-10T15:14:30Z","timestamp":1696950870000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1439-0388.2007.00645.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,6]]},"references-count":23,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2007,6]]}},"alternative-id":["10.1111\/j.1439-0388.2007.00645.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1439-0388.2007.00645.x","archive":["Portico"],"relation":{},"ISSN":["0931-2668","1439-0388"],"issn-type":[{"value":"0931-2668","type":"print"},{"value":"1439-0388","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,6]]}}}