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Activation of NF\u2010\u03baB induces production of pro\u2010inflammatory molecules including prostaglandin E2 (PGE<jats:sub>2<\/jats:sub>), interleukin\u20108 (IL\u20108) and macrophage chemotactic protein\u20101 (MCP\u20101). Nrf2 regulates genes controlling antioxidants. Our laboratory previously showed that hepatocytes, the primary functional cell type comprising liver tissue, respond to the cytokine interleukin\u20101 beta (IL\u20101\u03b2) by increased production of PGE<jats:sub>2<\/jats:sub>, IL\u20108 and MCP\u20101. This increase is associated with nuclear translocation of NF\u2010\u03baB. In this study, we evaluated whether primary canine hepatocytes pre\u2010treated with the combination of S\u2010adenosylmethionine (SAMe; 30 and 2000\u2003ng\/ml) and silybin (SB; 298\u2003ng\/ml), agents with known anti\u2010inflammatory and antioxidant properties, could attenuate IL\u20101\u03b2\u2010induced inflammation and oxidative stress. The SAMe and SB combination reduced cytokine\u2010induced PGE<jats:sub>2<\/jats:sub>, IL\u20108 and MCP\u20101 production while also inhibiting NF\u2010\u03baB nuclear translocation. These changes were accompanied by increased antioxidant enzyme\u2010reduced glutathione (GSH) comparable to control levels. The study shows for the first time that the SAMe and SB combination inhibits both inflammation and oxidative stress through two separate signalling pathways.<\/jats:p>","DOI":"10.1111\/j.1439-0396.2012.01275.x","type":"journal-article","created":{"date-parts":[[2012,2,9]],"date-time":"2012-02-09T17:59:30Z","timestamp":1328810370000},"page":"331-341","source":"Crossref","is-referenced-by-count":31,"title":["Hepatoprotective effects of S\u2010adenosylmethionine and silybin on canine hepatocytes <i>in vitro<\/i>"],"prefix":"10.1111","volume":"97","author":[{"given":"A. Y.","family":"Au","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. 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