{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T21:28:47Z","timestamp":1785446927738,"version":"3.56.0"},"reference-count":33,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2006,10,18]],"date-time":"2006-10-18T00:00:00Z","timestamp":1161129600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Veterinary Medicine Series A"],"published-print":{"date-parts":[[2006,11]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>This study aimed to evaluate stallion sperm survival after 24\u2003h of cooled storage in the presence of seminal plasma (SP) derived from the sperm\u2010rich fractions (SRF) or sperm\u2010poor fractions(SPF) of the ejaculate, without SP, or in the presence of SP from other stallions. Ejaculates were collected from four stallions using an automated phantom, which separated the semen into five cups. Centrifuged and washed spermatozoa from cup 2 (SRF) were mixed with skim milk extender to a concentration of 100\u2003\u00d7\u200310<jats:sup>6<\/jats:sup>\u2003sperm\/ml and then 1:1 (v\/v) with SP from the stallion's own or another stallions\u2019 second (SP\u2010SRF) or last cup (SP\u2010SPF). Skim milk extender (K) and skim milk extender supplemented with modified Tyrode's medium (KMT) were used as control treatments. After a 24\u2010h storage period in a transport container, spermatozoa were evaluated for motion characteristics and plasma membrane integrity by calcein acetoxymethyl (AM)\/propidium iodide staining. The percentage of spermatozoa with intact plasma membranes after storage was lower in SP\u2010SRF than in SP\u2010SPF, and the highest in K (<jats:italic>P<\/jats:italic>\u2003&lt;\u20030.05). Progressive motility (PMOT) was lower for sperm stored in SP\u2010SRF than for sperm stored in SP\u2010SPF (<jats:italic>P<\/jats:italic>\u2003&lt;\u20030.05), but there was no significant difference in total motility (TMOT). Sperm stored in KMT (<jats:italic>P<\/jats:italic>\u2003&lt;\u20030.05) registered the highest TMOT and PMOT percentages. Osmolarity was significantly higher and pH lower in K than in KMT or SP. Treatment with SP\u2010SPF from three stallions benefited the PMOT of sperm from one stallion. These preliminary findings suggest that SP from SRFs may be more harmful during storage than SP from SPFs. Removal of SP improves sperm survival in KMT extender, and exchanging SP between stallions seems to influence sperm survival.<\/jats:p>","DOI":"10.1111\/j.1439-0442.2006.00882.x","type":"journal-article","created":{"date-parts":[[2006,10,18]],"date-time":"2006-10-18T12:05:10Z","timestamp":1161173110000},"page":"481-485","source":"Crossref","is-referenced-by-count":26,"title":["Effect of Seminal Plasma Fractions on Stallion Sperm Survival after Cooled Storage"],"prefix":"10.1111","volume":"53","author":[{"given":"E.","family":"Akcay","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Reilas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Andersson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Katila","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,10,18]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-4320(93)90095-9"},{"key":"e_1_2_6_3_1","first-page":"113","article-title":"Proteins in seminal plasma","volume":"35","author":"Amann R. 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