{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T15:46:52Z","timestamp":1759938412604},"reference-count":66,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2007,8,20]],"date-time":"2007-08-20T00:00:00Z","timestamp":1187568000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Veterinary Medicine Series A"],"published-print":{"date-parts":[[2007,9]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>In this study, we investigated the susceptibility of frozen\u2013thawed swamp buffalo sperm nuclear DNA to undergo controlled acid\u2010induced denaturation <jats:italic>in situ<\/jats:italic>, as analysed by flow cytometry, and aimed to correlate the results with sperm head morphology over three seasons in tropical Thailand. Artificial insemination (AI) doses (<jats:italic>n<\/jats:italic>\u2003=\u2003218) from 18 AI buffalo sires, prepared between 1980 and 1989 and 2003 and 2005, were tested and compared among three seasons, the rainy season, July\u2013October; winter, November\u2013February; and summer, March\u2013June. The overall mean of DNA fragmentation index (DFI) (\u00b1 SD) was 1.84\u2003\u00b1\u20031.68%, range from 0.19 to 7.92%, with 0.221\u2003\u00b1\u20030.021 of the <jats:italic>x<\/jats:italic>\u2010DFI ranging from 0.190 to 0.350 and 0.023\u2003\u00b1\u20030.009 of the SD\u2010DFI ranging from 0.010 to 0.070. The DFI was consistently low (range 1.40\u2003\u00b1\u20030.21% to 2.16\u2003\u00b1\u20030.21%; LSM\u2003\u00b1\u2003SEM), with x\u2010DFI ranging from 0.216\u2003\u00b1\u20030.003 to 0.225\u2003\u00b1\u20030.003 and SD\u2010DFI ranging from 0.022\u2003\u00b1\u20030.001 to 0.024\u2003\u00b1\u20030.001 across the seasons. The DFI was low enough to be related to high fertility potential. However, DFI values varied statistically among seasons, being lower in the rainy season (1.40\u2003\u00b1\u20030.21%, <jats:italic>P\u2003<\/jats:italic>&lt;\u20030.05) than in winter (2.16\u2003\u00b1\u20030.21%) or summer (2.00\u2003\u00b1\u20030.20%), and were also affected by the year of semen collection and processing (<jats:italic>P\u2003<\/jats:italic>&lt;\u20030.001). The proportion of morphologically abnormal sperm head shapes was low, with no significant differences between seasons. However, DFI was significantly related to the proportion of loose abnormal sperm heads (<jats:italic>r<\/jats:italic>\u2003=\u20030.27, <jats:italic>P\u2003<\/jats:italic>&lt;\u20030.01). In conclusion, frozen\u2013thawed swamp buffalo sperm chromatin integrity is not seriously damaged by cryopreservation or affected by the seasonal variations in temperature and humidity seen in tropical Thailand.<\/jats:p>","DOI":"10.1111\/j.1439-0442.2007.00946.x","type":"journal-article","created":{"date-parts":[[2007,8,21]],"date-time":"2007-08-21T03:46:06Z","timestamp":1187667966000},"page":"377-383","source":"Crossref","is-referenced-by-count":10,"title":["Seasonal Variation in Nuclear DNA Integrity of Frozen\u2013Thawed Spermatozoa from Thai AI Swamp Buffaloes (<i>Bubalus bubalis<\/i>)"],"prefix":"10.1111","volume":"54","author":[{"given":"S.","family":"Koonjaenak","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Johannisson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Pongpeng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Wirojwuthikul","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Kunavongkrit","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Rodriguez\u2010Martinez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,8,20]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(01)00599-4"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod66.2.354"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1006\/excr.1997.3719"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01047289"},{"key":"e_1_2_6_6_1","doi-asserted-by":"crossref","first-page":"995","DOI":"10.2527\/jas1986.633995x","article-title":"Flow cytometry evaluation of testicular and sperm cells obtained from bulls implanted with zeranol","volume":"63","author":"Ballachey B. 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