{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,1,26]],"date-time":"2024-01-26T22:24:08Z","timestamp":1706307848348},"reference-count":20,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2007,11,27]],"date-time":"2007-11-27T00:00:00Z","timestamp":1196121600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Veterinary Medicine Series A"],"published-print":{"date-parts":[[2007,12]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Avian leukosis virus (ALV) subgroup J (ALV\u2010J) is an exogenous ALV and causes myeloid leukosis in meat\u2010type chickens. We have previously reported the isolation and identification of ALV\u2010J in commercial layer flocks from 12 farms in northern China. In this report, we further characterized this virus by <jats:italic>in situ<\/jats:italic> polymerase chain reaction (PCR) hybridization in various affected organs of chickens from six of the 12 farms. A routine method for hybridization of nucleic acid uses radioactive probe, such as a P<jats:sup>32<\/jats:sup>\u2010labelled probe. We found that the non\u2010radioactive digoxigenin (DIG) probe is sensitive enough to detect the nucleic acid of virus in chicken tissues. We used a pair of published primers (H5\/H7) specific to the gp85 envelope gene and 3\u2032 region of <jats:italic>pol<\/jats:italic> gene of prototype ALV\u2010J strain HPRS\u2010103. The total RNA extracted from tumour, bone marrow, oviduct, liver and spleen of the diseased chickens from six commercial flocks, and cDNA was successfully amplified. Using the primers and cDNA, we obtained an ALV\u2010J\u2010specific cDNA probe of 545\u2003bp in length by PCR. <jats:italic>In situ<\/jats:italic> PCR with H5\/H7 primers was carried out in the paraffin sections from tissues of the diseased chickens, followed by <jats:italic>in situ<\/jats:italic> hybridization using the DIG\u2010labelled cDNA probe. Positive hybridization signals were detected in the cytoplasm of paraffin sections of tumours and other organ tissues. The intensity of the signals was documented using an image analysis system measuring integral optical density (IOD). The IOD values for tissue sections treated by <jats:italic>in situ<\/jats:italic> PCR hybridization are significantly higher than that by <jats:italic>in situ<\/jats:italic> hybridization alone (<jats:italic>P<\/jats:italic>\u2003&lt;\u20030.01). These data taken together suggest that <jats:italic>in situ<\/jats:italic> PCR hybridization is a more sensitive technique for detection of ALV\u2010J in tissue sections.<\/jats:p>","DOI":"10.1111\/j.1439-0442.2007.01008.x","type":"journal-article","created":{"date-parts":[[2007,12,4]],"date-time":"2007-12-04T00:37:58Z","timestamp":1196728678000},"page":"553-558","source":"Crossref","is-referenced-by-count":6,"title":["Detection and Localization of Naturally Transmitted Avian Leukosis Subgroup J Virus in Egg\u2010type Chickens by <i>In situ<\/i> PCR Hybridization"],"prefix":"10.1111","volume":"54","author":[{"given":"N.","family":"Li","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.","family":"Xu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W.","family":"Dong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Qiao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L. F.","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. M.","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N.","family":"Du","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,11,27]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.2307\/1592351"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1080\/03079459994894"},{"key":"e_1_2_6_4_1","first-page":"404","volume-title":"Applied Immunocytochemistry and Nucleic Acid Molecule Hybridization Technology","author":"Cai W.","year":"1994"},{"key":"e_1_2_6_5_1","first-page":"584","article-title":"Emerging of avian leukosis virus subgroup J in a flock of Chinese local breed","volume":"45","author":"Cheng Z. Q.","year":"2005","journal-title":"Wei. Sheng. Wu. Xue. 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Med."},{"key":"e_1_2_6_20_1","first-page":"562","article-title":"Pathology diagnosis of avian myelocytomatosis from egg\u2010type chickens","volume":"33","author":"Xu B.","year":"2002","journal-title":"Chin. J. Anim. Vet. Sci."},{"key":"e_1_2_6_21_1","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1080\/03079450310001636237a","article-title":"Occurrence of avian leukosis virus subgroup J in commercial layer flocks in China","volume":"33","author":"Xu B.","year":"2004","journal-title":"Avian Pathol."}],"container-title":["Journal of Veterinary Medicine Series A"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1439-0442.2007.01008.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1439-0442.2007.01008.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,14]],"date-time":"2023-10-14T04:17:14Z","timestamp":1697257034000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1439-0442.2007.01008.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,11,27]]},"references-count":20,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2007,12]]}},"alternative-id":["10.1111\/j.1439-0442.2007.01008.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1439-0442.2007.01008.x","archive":["Portico"],"relation":{},"ISSN":["0931-184X"],"issn-type":[{"value":"0931-184X","type":"print"}],"subject":[],"published":{"date-parts":[[2007,11,27]]}}}