{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T04:22:08Z","timestamp":1790482928532,"version":"4.1.0"},"reference-count":6,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2004,4,23]],"date-time":"2004-04-23T00:00:00Z","timestamp":1082678400000},"content-version":"vor","delay-in-days":22,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Veterinary Medicine, Series B"],"published-print":{"date-parts":[[2004,4]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Forty\u2010four faecal samples from piglets (2\u20133 animals per group) experimentally infected with <jats:italic>Isospora suis<\/jats:italic> were examined 4, 5, 7, 10, and 11\u2003days after infection either with a modified flotation technique followed by McMaster counting (MM), MM combined with autofluorescence microscopy of faecal smears (MM\u2010A), or nested\u2010polymerase chain reaction (PCR). PCR was the most sensitive method, detecting <jats:italic>I. suis<\/jats:italic> in 70% of the samples (MM 25%, MM\u2010A 52%). More positive samples were detected in the untreated and the diclazuril\u2010treated [15\u2003mg\/kg bodyweight (BW) once or twice, respectively] groups (10 samples per group; MM 30\u201340%, MM\u2010A 50\u201370%, PCR 60\u201390%), than in the toltrazuril\u2010treated (20\u2003mg\/kg BW) group (14 samples; MM 0%, MM\u2010A 21%, PCR 57%). Parasites could be detected in the untreated group on all days with PCR and MM\u2010A, while MM detected oocysts only from day 5. Autofluorescence should be used as a routine diagnostic aid for the detection of oocysts in faecal samples, while PCR, being the most sensitive technique, can be employed when large numbers of samples are examined, and in experimental setups.<\/jats:p>","DOI":"10.1111\/j.1439-0450.2004.00736.x","type":"journal-article","created":{"date-parts":[[2004,4,23]],"date-time":"2004-04-23T08:25:33Z","timestamp":1082708733000},"page":"140-142","source":"Crossref","is-referenced-by-count":12,"title":["Detection of <i>Isospora suis<\/i> (Biester and Murray 1934) in Piglet Faeces \u2013 Comparison of Microscopy and PCR"],"prefix":"10.1111","volume":"51","author":[{"given":"A.","family":"Joachim","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Ruttkowski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Zimmermann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Daugschies","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.\u2010C.","family":"Mundt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2004,4,23]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004360100378"},{"key":"e_1_2_5_3_1","first-page":"259","article-title":"Studies on preweaning piglet diarrhoea","volume":"70","author":"Driesen S. J.","year":"1993","journal-title":"Vet. J."},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1080\/01652176.1983.9693894"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-4017(99)00027-8"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1439-0450.2002.00459.x"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-4017(00)00408-8"}],"container-title":["Journal of Veterinary Medicine, Series B"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1439-0450.2004.00736.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1439-0450.2004.00736.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,10]],"date-time":"2023-10-10T16:20:12Z","timestamp":1696954812000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1439-0450.2004.00736.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,4]]},"references-count":6,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2004,4]]}},"alternative-id":["10.1111\/j.1439-0450.2004.00736.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1439-0450.2004.00736.x","archive":["Portico"],"relation":{},"ISSN":["0931-1793"],"issn-type":[{"value":"0931-1793","type":"print"}],"subject":[],"published":{"date-parts":[[2004,4]]}}}