{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,1,10]],"date-time":"2024-01-10T12:56:11Z","timestamp":1704891371040},"reference-count":15,"publisher":"Wiley","issue":"S1","license":[{"start":{"date-parts":[[2011,4,14]],"date-time":"2011-04-14T00:00:00Z","timestamp":1302739200000},"content-version":"vor","delay-in-days":3147,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2002,9]]},"abstract":"<jats:p><jats:bold>Zusammenfassung. <\/jats:bold> Mit den klassischen mikrobiologischen Verfahren ist eine eindeutige Identifizierung der bislang einzigen klinisch relevanten <jats:italic>Cunninghamella<\/jats:italic>\u2010Art, <jats:italic>C. bertholletiae<\/jats:italic>, nicht m\u00f6glich. Mittels PCR und Sequenzierung der Internal\u2010transcribed\u2010spacer\u2010(ITS)\u2010DNA\u2010Region konnten von neun klinischen Isolaten der Gattung <jats:italic>Cunninghamella<\/jats:italic> sieben als <jats:italic>C. bertholletiae<\/jats:italic> und zwei als <jats:italic>C. echinulata<\/jats:italic> identifiziert werden. Ein Umgebungsisolat eines mit <jats:italic>C. echinulata<\/jats:italic> infizierten Patienten konnte ebenfalls als <jats:italic>C. echinulata<\/jats:italic> identifiziert werden. Alle Isolate der Spezies <jats:italic>C. bertholletiae<\/jats:italic> zeigten eine hohe Sequenzhomologie der ITS\u2010Region. Innerhalb der Spezies <jats:italic>C. echinulata<\/jats:italic> und <jats:italic>C. elegans<\/jats:italic> konnte aufgrund unterschiedlicher Restriktionsfragmentl\u00e4ngen\u2010Profile nach Inkubation der ITS\u2010Amplifikate mit <jats:italic>Taq<\/jats:italic>I und <jats:italic>Hinf<\/jats:italic>I eine Subspeziesdifferenzierung erreicht werden. \u00c4hnliche Ergebnisse wurden auch mittels PCR fingerprinting der Gesamt\u2010DNA mit den Microsatellit\u2010DNA\u2010Primern (GTG)<jats:sub>5<\/jats:sub> and (GAC)<jats:sub>5<\/jats:sub> erhalten. Erstmalig konnte <jats:italic>C. echinulata<\/jats:italic> als Erreger einer Zygomykose beim Menschen identifiziert werden.<\/jats:p><jats:p><jats:bold>Summary. <\/jats:bold> Among the genus <jats:italic>Cunninghamella<\/jats:italic>, so far <jats:italic>C. bertholletiae<\/jats:italic> is known to be the only clinically relevant species. Correct identification of <jats:italic>C. bertholletiae<\/jats:italic> is not possible with classical methods. PCR and sequencing of the internal transcribed spacer (ITS) region was used to identify seven of nine clinical isolates as <jats:italic>C. bertholletiae<\/jats:italic> and two as <jats:italic>C. echinulata.<\/jats:italic> Also an isolate of the surrounding area of one patient infected with <jats:italic>C. echinulata<\/jats:italic> could be identified as <jats:italic>C. echinulata.<\/jats:italic> High homology in the ITS region was found within the isolates of <jats:italic>C. bertholletiae.<\/jats:italic> Within the species <jats:italic>C. echinulata<\/jats:italic> and <jats:italic>C. elegans<\/jats:italic> a differentiation on subspecies level was achieved by an analysis of restriction fragment length polymorphism of the ITS amplicons after incubation with <jats:italic>Taq<\/jats:italic>I and <jats:italic>Hinf<\/jats:italic>I. Similar results were obtained by PCR fingerprinting of the complete DNA with the single microsatellite DNA primers (GTG)<jats:sub>5<\/jats:sub> and (GAC)<jats:sub>5<\/jats:sub>. For the first time <jats:italic>C. echinulata<\/jats:italic> could be identified as agent of zygomycosis in humans.<\/jats:p>","DOI":"10.1111\/j.1439-0507.2002.tb04543.x","type":"journal-article","created":{"date-parts":[[2011,4,14]],"date-time":"2011-04-14T10:02:18Z","timestamp":1302775338000},"page":"31-36","source":"Crossref","is-referenced-by-count":13,"title":["Molekularbiologische Identifizierung von <i>Cunninghamella<\/i> spec."],"prefix":"10.1111","volume":"45","author":[{"given":"Karin","family":"Lemmer","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heidemarie","family":"Losert","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V.","family":"Rickerts","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gudrun","family":"Just\u2010N\u00fcbling","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"Sander","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marie\u2010Louise","family":"Kerkmann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kathrin","family":"Tintelnot","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2011,4,14]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0507.1993.tb00769.x"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1086\/313537"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1086\/318144"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0007-1536(84)80056-X"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1080\/mmy.38.6.437.441"},{"key":"e_1_2_1_7_2","first-page":"194","article-title":"rDNA diversity and physiology of Ochroconis and Scolecobasidium species reported from humans and other vertebrates","volume":"43","author":"Horr\u00e9 R.","year":"1999","journal-title":"Stud. Mycol."},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.11501601273"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid0701.010123"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1080\/mmy.40.2.213.216"},{"key":"e_1_2_1_11_2","first-page":"315","volume-title":"PCR Protocols: A guide to methods and applications","author":"White T.J.","year":"1990"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.12.3957-3964.1999"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.74.12.5463"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00873289"},{"key":"e_1_2_1_15_2","first-page":"151","article-title":"Variability and molecular diagnostics of the neurotropic species Cladophialophora bantiana","volume":"43","author":"Ende Gerrits A.H.G.","year":"1999","journal-title":"Stud. Mycol."},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0953756298007667"}],"container-title":["Mycoses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1439-0507.2002.tb04543.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1439-0507.2002.tb04543.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,18]],"date-time":"2023-11-18T13:41:38Z","timestamp":1700314898000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1439-0507.2002.tb04543.x"}},"subtitle":["Identification of <i>Cunninghamella<\/i> spec. by molecular methods"],"short-title":[],"issued":{"date-parts":[[2002,9]]},"references-count":15,"journal-issue":{"issue":"S1","published-print":{"date-parts":[[2002,9]]}},"alternative-id":["10.1111\/j.1439-0507.2002.tb04543.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1439-0507.2002.tb04543.x","archive":["Portico"],"relation":{},"ISSN":["0933-7407","1439-0507"],"issn-type":[{"value":"0933-7407","type":"print"},{"value":"1439-0507","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,9]]}}}