{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,11]],"date-time":"2023-10-11T05:07:44Z","timestamp":1697000864922},"reference-count":17,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2004,8,12]],"date-time":"2004-08-12T00:00:00Z","timestamp":1092268800000},"content-version":"vor","delay-in-days":11,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2004,8]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Reconstituted multilayered oesophageal epithelium appears to be a good basis to test the efficacy of voriconazole (VOR) and fluconazole (FLU) in the tissue. The resulting model of a <jats:italic>Candida<\/jats:italic> oesophagitis was approaching the <jats:italic>in vivo<\/jats:italic> situation. We infected the tissue with 2\u2003\u00d7\u200310<jats:sup>6<\/jats:sup>\u2003cfu of the <jats:italic>Candida albicans<\/jats:italic> strain SC5314. In the trials with FLU we also used clinical strains. Four hours after infection a good growth of <jats:italic>C. albicans<\/jats:italic> appeared mainly with hyphae on the surface of the tissue and a tendency to invasion. The destruction of the tissue began after 36\u2003h. VOR (2 and 16\u2003<jats:italic>\u03bc<\/jats:italic>g\u2003ml<jats:sup>\u22121<\/jats:sup>, respectively) prevented the penetration of hyphae into the tissue, when it was given 4\u20138\u2003h after infection. It was less effective in reduction of <jats:italic>Candida<\/jats:italic> growth on the tissue surface. When VOR was given 16\u201324\u2003h postinfection, the <jats:italic>Candida<\/jats:italic> infiltration stopped more slowly. Thirty\u2010six hours after infection VOR application could not stop the destruction of the tissue despite reducing the fungi. The results with FLU (32\u2003<jats:italic>\u03bc<\/jats:italic>g\u2003ml<jats:sup>\u22121<\/jats:sup>) were in principle the same, but not so distinct. FLU seems to be more effective against clinical strains of <jats:italic>C. albicans<\/jats:italic> than against the type strain.<\/jats:p>","DOI":"10.1111\/j.1439-0507.2004.01004.x","type":"journal-article","created":{"date-parts":[[2004,8,12]],"date-time":"2004-08-12T10:31:24Z","timestamp":1092306684000},"page":"330-337","source":"Crossref","is-referenced-by-count":5,"title":["Influence of voriconazole and fluconazole on reconstituted multilayered oesophageal epithelium infected by <i>Candida albicans<\/i>"],"prefix":"10.1111","volume":"47","author":[{"given":"J.","family":"Bernhardt","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Bernhardt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Knoke","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Ludwig","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,8,12]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1086\/323807"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.3.1558-1565.2002"},{"key":"e_1_2_6_4_2","first-page":"408","volume-title":"42nd Interscience Conference on Antimicrobial Agents and Chemotherapy","author":"Bernhardt J","year":"2002"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1099\/13500872-142-10-2741"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1998.00957.x"},{"issue":"2","key":"e_1_2_6_7_2","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1111\/j.1439-0507.1999.tb00009.x","article-title":"The continuous flow culture as an in vitro model in experimental mycology","volume":"42","author":"Bernhardt H","year":"1999","journal-title":"Mycoses"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0507.2002.tb04545.x"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1010954803886"},{"key":"e_1_2_6_10_2","first-page":"411","volume-title":"42nd Interscience Conference on Antimicrobial Agents and Chemotherapy","author":"Johnson E","year":"2002"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1007\/s100960050313"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1358\/dot.2001.37.2.614849"},{"key":"e_1_2_6_13_2","doi-asserted-by":"crossref","first-page":"1840","DOI":"10.1128\/AAC.41.8.1840","article-title":"Voriconazole (UK\u2010109,496) inhibits the growth and alters the morphology of fluconazole\u2010susceptible and \u2010resistant Candida species","volume":"41","author":"Belanger P","year":"1997","journal-title":"Antimicrob Agents Chemother"},{"key":"e_1_2_6_14_2","first-page":"393","volume-title":"42nd Interscience Conference on Antimicrobial Agents and Chemotherapy","author":"Waldrep TW","year":"2002"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.12.1.40"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.44.7.1917-1920.2000"},{"key":"e_1_2_6_17_2","first-page":"408","volume-title":"42nd Interscience Conference on Antimicrobial Agents and Chemotherapy","author":"Bernhardt H","year":"2002"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1086\/322653"}],"container-title":["Mycoses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1439-0507.2004.01004.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1439-0507.2004.01004.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,10]],"date-time":"2023-10-10T18:51:49Z","timestamp":1696963909000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1439-0507.2004.01004.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,8]]},"references-count":17,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2004,8]]}},"alternative-id":["10.1111\/j.1439-0507.2004.01004.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1439-0507.2004.01004.x","archive":["Portico"],"relation":{},"ISSN":["0933-7407","1439-0507"],"issn-type":[{"value":"0933-7407","type":"print"},{"value":"1439-0507","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,8]]}}}