{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T03:53:25Z","timestamp":1777348405865,"version":"3.51.4"},"reference-count":18,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2006,4,25]],"date-time":"2006-04-25T00:00:00Z","timestamp":1145923200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2006,5]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>The basidiomycetous yeast <jats:italic>Filobasidium capsuligenum<\/jats:italic> produces a killer toxin (FC\u20101) which is highly effective against the opportunistic fungal pathogen <jats:italic>Cryptococcus neoformans<\/jats:italic>. The aim of this work was to study the effect of the toxin on <jats:italic>C. neoformans<\/jats:italic> cells. The sensitivities of strains representing eight molecular subtypes (VNI\u2013IV and VGI\u2013IV) of the <jats:italic>C. neoformans<\/jats:italic> species complex, and of an additional 50 clinical and environmental isolates were determined. Analysis of cellular DNA by laser scanning cytometry and fluorescein isothiocyanate (FITC) staining of the toxin\u2010treated cells revealed that the killing mechanism of FC\u20101 is neither cell cycle\u2010 nor cell wall biosynthesis\u2010dependent; rather it may act as an ionophoric protein that disrupts the cytoplasmic membrane function. The competition assay results suggest that <jats:italic>\u03b2<\/jats:italic>\u20101,6\u2010glucan in the cell wall may provide the binding site for the killer protein. This anticryptococcal toxin has the potential to be applied as a therapeutic agent for the treatment of cryptococcosis.<\/jats:p>","DOI":"10.1111\/j.1439-0507.2006.01227.x","type":"journal-article","created":{"date-parts":[[2006,4,25]],"date-time":"2006-04-25T16:59:27Z","timestamp":1145984367000},"page":"176-183","source":"Crossref","is-referenced-by-count":17,"title":["Characterisation of the anticryptococcal effect of the FC\u20101 toxin produced by <i>Filobasidium capsuligenum<\/i>"],"prefix":"10.1111","volume":"49","author":[{"given":"A.","family":"Keszthelyi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Ohkusu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Takeo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I.","family":"Pfeiffer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Litter","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Kucsera","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,4,25]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.8.4.515"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0924-8579(95)00046-1"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt0297-155"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-695X.1998.tb01200.x"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkf040"},{"key":"e_1_2_6_7_2","first-page":"530","article-title":"The taxonomic specificity of the action spectrum of Filobasidium capsuligenum mycocin","volume":"60","author":"Golubev WI","year":"1991","journal-title":"Microbiology [in Russian]"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1080\/02681219780001051"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1439-0507.1999.00528.x"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02328080"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00407768"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2001.tb09478.x"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1567-1356(03)00176-4"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/EC.2.4.655-663.2003"},{"key":"e_1_2_6_15_2","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1128\/jb.154.1.161-169.1983","article-title":"Cell wall receptor for yeast killer toxin: involvement of (1\u20106)\u2010\u03b2\u2010D\u2010glucan","volume":"154","author":"Hutchins K","year":"1983","journal-title":"J Bacteriol"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-142-9-2655"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00438-003-0920-5"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.2002.tb00614.x"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2004.10.015"}],"container-title":["Mycoses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1439-0507.2006.01227.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1439-0507.2006.01227.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,27]],"date-time":"2023-09-27T01:05:29Z","timestamp":1695776729000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1439-0507.2006.01227.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,4,25]]},"references-count":18,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2006,5]]}},"alternative-id":["10.1111\/j.1439-0507.2006.01227.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1439-0507.2006.01227.x","archive":["Portico"],"relation":{},"ISSN":["0933-7407","1439-0507"],"issn-type":[{"value":"0933-7407","type":"print"},{"value":"1439-0507","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,4,25]]}}}