{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,9]],"date-time":"2025-04-09T06:03:52Z","timestamp":1744178632322},"reference-count":23,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2006,4,25]],"date-time":"2006-04-25T00:00:00Z","timestamp":1145923200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2006,5]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p> <jats:italic>Paracoccidioides brasiliensis<\/jats:italic> is the agent of paracoccidioidomycosis, the most prevalent mycosis in Latin America, and currently there is no effective vaccine. The aim of this study was to attenuate the yeast form of <jats:italic>P. brasiliensis<\/jats:italic> by gamma irradiation for further studies on vaccine research. <jats:italic>Paracoccidioides brasiliensis<\/jats:italic> (strain Pb 18) cultures were irradiated at doses between 0.5 and 8.0\u2003kGy. After each dose the viability, reproductive ability and protein metabolism were evaluated. The comparison between the antigenic profile of irradiated and control yeast was made by Western blot and the virulence evaluated by the inoculation in C<jats:sub>57<\/jats:sub>Bl\/J6 mice. At 6.5\u2003kGy the yeast lost its reproductive capacity. The viability and the incorporation of [L\u2010<jats:sup>35<\/jats:sup>S]\u2010methionine were the same in control and up to 6.5\u2003kGy irradiated cells, but 6.5\u2003kGy\u2010irradiated yeast secreted 40% less proteins. The Western blot profile was clearly similar in control and 6.5\u2003kGy\u2010irradiated yeast. No colony\u2010forming unit (CFU) could be recovered from the tissues of the mice infected with the radioattenuated yeast. We concluded that for <jats:italic>P. brasiliensis<\/jats:italic> yeast it is possible to find a dose in which the pathogen loses its reproductive ability and virulence, while retaining its viability, metabolic activity and the antigenic profile.<\/jats:p>","DOI":"10.1111\/j.1439-0507.2006.01229.x","type":"journal-article","created":{"date-parts":[[2006,4,25]],"date-time":"2006-04-25T16:59:27Z","timestamp":1145984367000},"page":"184-189","source":"Crossref","is-referenced-by-count":11,"title":["<i>Paracoccidioides brasiliensis<\/i>: attenuation of yeast cells by gamma irradiation"],"prefix":"10.1111","volume":"49","author":[{"given":"Marina Cortez","family":"Demicheli","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bernardo Sgarbi","family":"Reis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alfredo Miranda","family":"Goes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antero Silva","family":"Ribeiro de Andrade","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,4,25]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.6.2.89"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0966-842X(01)02292-2"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.0300-9475.2004.01359.x"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2003.07.017"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-308X(08)60048-2"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(02)00054-3"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.9.4066"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3024.1993.tb00587.x"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1086\/515301"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.2307\/1592164"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4017(86)90093-2"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/0169-4758(92)90167-Z"},{"key":"e_1_2_6_14_2","doi-asserted-by":"crossref","first-page":"3383","DOI":"10.1128\/IAI.67.7.3383-3389.1999","article-title":"Isolation of T cell antigens by using a recombinant protein library and its application to the identification of novel vaccine candidates against schistosomiasis","volume":"67","author":"Eberl M","year":"1999","journal-title":"Infect Immun"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1086\/339409"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1136\/vr.119.15.370"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01103733"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2621.1941.tb16295.x"},{"key":"e_1_2_6_19_2","first-page":"22","volume-title":"Food Irradiation: Principles and Applications","author":"Dickson DS.","year":"2001"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.62.4.1465-1469.1994"},{"key":"e_1_2_6_21_2","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1042\/bj3090209","article-title":"Characterization of an exocellular serine\u2010thiol proteinase activity in Paracoccidioides brasiliensis","volume":"30","author":"Carmona AK","year":"1995","journal-title":"Biochem J"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.54.8.2134-2135.1988"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0955-0674(98)80118-X"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.2307\/3575641"}],"container-title":["Mycoses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1439-0507.2006.01229.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1439-0507.2006.01229.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,27]],"date-time":"2023-09-27T01:05:37Z","timestamp":1695776737000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1439-0507.2006.01229.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,4,25]]},"references-count":23,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2006,5]]}},"alternative-id":["10.1111\/j.1439-0507.2006.01229.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1439-0507.2006.01229.x","archive":["Portico"],"relation":{},"ISSN":["0933-7407","1439-0507"],"issn-type":[{"value":"0933-7407","type":"print"},{"value":"1439-0507","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,4,25]]}}}