{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,28]],"date-time":"2025-04-28T16:30:14Z","timestamp":1745857814554},"reference-count":19,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2007,5,2]],"date-time":"2007-05-02T00:00:00Z","timestamp":1178064000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2007,9]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p> <jats:italic>Paracoccidioides brasiliensis<\/jats:italic> is a thermally dimorphic fungus agent of paracoccidioidomycosis, a deep\u2010seated systemic infection of humans with high prevalence in Latin America. Until now no vaccine has been reported. Ionizing radiation can be used to attenuate pathogens for vaccine development and we have successfully attenuated yeast cells of <jats:italic>P. brasiliensis<\/jats:italic> by gamma irradiation. The aim of the present study was to examine at ultrastructural level the effects of gamma irradiation attenuation on the morphology of <jats:italic>P. brasiliensis<\/jats:italic> yeast cells. <jats:italic>Paracoccidioides brasiliensis<\/jats:italic> (strain Pb\u201018) cultures were irradiated with a dose of 6.5\u2003kGy. The irradiated cells were examined by scanning and also transmission electron microscopy. When examined 2\u2003h after the irradiation by scanning electron microscopy, the 6.5\u2003kGy irradiated cells presented deep folds or were collapsed. These lesions were reversible since when examined 48\u2003h after irradiation the yeast had recovered the usual morphology. The transmission electron microscopy showed that the irradiated cells plasma membrane and cell wall were intact and preserved. Remarkable changes were found in the nucleus that was frequently in a very electrondense form. An extensive DNA fragmentation was produced by the gamma irradiation treatment.<\/jats:p>","DOI":"10.1111\/j.1439-0507.2007.01389.x","type":"journal-article","created":{"date-parts":[[2007,5,9]],"date-time":"2007-05-09T02:36:33Z","timestamp":1178678193000},"page":"397-402","source":"Crossref","is-referenced-by-count":12,"title":["Ultrastructural changes in <i>Paracoccidioides brasiliensis<\/i> yeast cells attenuated by gamma irradiation"],"prefix":"10.1111","volume":"50","author":[{"given":"Marina Cortez","family":"Demicheli","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alfredo Miranda","family":"Goes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antero Silva Ribeiro","family":"De Andrade","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,5,2]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.6.2.89"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.0300-9475.2004.01359.x"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2003.07.017"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1136\/vr.119.15.370"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0169-4758(92)90167-Z"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0507.2006.01229.x"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01103733"},{"key":"e_1_2_6_9_2","first-page":"27","volume-title":"Paracoccidioidomycosis","author":"Queiroz\u2010Telles F","year":"1994"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tiv.2003.08.011"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0955-0674(98)80118-X"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.2307\/3575641"},{"key":"e_1_2_6_13_2","first-page":"43","article-title":"Structure and development of normal and X\u2010irradiated yeast protoplasts","volume":"6","author":"Meissel M","year":"1966","journal-title":"Abh Deul Akad Wiss Berlin"},{"key":"e_1_2_6_14_2","first-page":"243","article-title":"Gamma Strahlen auf die subzellulare Struktur von Rhodotorula","volume":"6","author":"Paschev I","year":"1966","journal-title":"Abh Deul Akad Wiss Berlin"},{"key":"e_1_2_6_15_2","first-page":"36","article-title":"The fine structure of normal and irradiated yeast cells and yeast ribosomes","volume":"12","author":"Koehler JK","year":"1961","journal-title":"Univ Calif Rad Lab UCRL-9715"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02051669"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1006\/fgbi.1998.1093"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-5699(93)90214-6"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1567-1356(03)00154-5"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0968-4328(99)00083-9"}],"container-title":["Mycoses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1439-0507.2007.01389.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1439-0507.2007.01389.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,9]],"date-time":"2023-10-09T19:42:05Z","timestamp":1696880525000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1439-0507.2007.01389.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,5,2]]},"references-count":19,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2007,9]]}},"alternative-id":["10.1111\/j.1439-0507.2007.01389.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1439-0507.2007.01389.x","archive":["Portico"],"relation":{},"ISSN":["0933-7407","1439-0507"],"issn-type":[{"value":"0933-7407","type":"print"},{"value":"1439-0507","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,5,2]]}}}