{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T14:16:57Z","timestamp":1773325017578,"version":"3.50.1"},"reference-count":19,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2008,4,16]],"date-time":"2008-04-16T00:00:00Z","timestamp":1208304000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2008,7]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Dermatophytoses caused by the genus <jats:italic>Microsporum<\/jats:italic> require a long\u2010duration therapy compared to infections caused by other genera. Treatment of these cutaneous infections includes topical and systemic antifungal agents. <jats:italic>Tinea capitis<\/jats:italic> and <jats:italic>tinea unguium<\/jats:italic> caused by <jats:italic>M. canis<\/jats:italic> and <jats:italic>M. gypseum<\/jats:italic> are the most difficult\u2010to\u2010treat dermatophytoses. There are few specific studies about corresponding antifungal susceptibility <jats:italic>in vitro.<\/jats:italic> Recently, the Clinical and Laboratory Standards Institute proposed the M38A document as standard to determine the minimum inhibitory concentrations (MICs) of several antifungal agents against conidium\u2010forming filamentous fungi; however, dermatophytes were not included in this document. This study aimed to contribute to continuing investigations concerning the optimal antifungal susceptibility testing conditions of <jats:italic>Microsporum<\/jats:italic> spp. to terbinafine, ciclopiroxolamine and griseofulvin<jats:italic>.<\/jats:italic> The results pointed out potato dextrose agar as the best culture medium for inducing conidia sporulation, inoculum density amounting to 1\u2003\u00d7\u200310<jats:sup>3<\/jats:sup>\u2003conidia\u2003ml<jats:sup>\u22121<\/jats:sup>, containing only microconidia, with an incubation time of 7\u2003days at 28\u2003\u00b0C and 100% growth inhibition serving as an endpoint. The minimum fungicidal concentration values were in accordance with the MICs values, showing a fungicidal activity of these drugs towards the tested strains. According to our results, in general, terbinafine was more active than griseofulvin and ciclopiroxolamine.<\/jats:p>","DOI":"10.1111\/j.1439-0507.2008.01503.x","type":"journal-article","created":{"date-parts":[[2008,4,17]],"date-time":"2008-04-17T06:46:52Z","timestamp":1208414812000},"page":"313-317","source":"Crossref","is-referenced-by-count":6,"title":["Preanalytical conditions for broth microdilution antifungal susceptibility of <i>Microsporum<\/i> spp."],"prefix":"10.1111","volume":"51","author":[{"given":"Fernanda Simas","family":"Corr\u00eaa Biancalana","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paula Fernanda Gomes","family":"Telles","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luzia","family":"Lyra","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ang\u00e9lica Zaninelli","family":"Schreiber","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2008,4,16]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11046-005-0252-6"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1097\/00001432-200404000-00005"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0507.2004.01024.x"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2133.2002.04744.x"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.1.385-388.2001"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2133.2003.05418.x"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.10.4817-4819.2003"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.45.9.2524-2528.2001"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.11.3999-4003.2002"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1080\/13693780500092947"},{"key":"e_1_2_6_12_2","volume-title":"Reference Method for Broth Dilution Antifungal Susceptibility Testing of Conidium\u2010Forming Filamentous Fungi. 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