{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T11:39:53Z","timestamp":1787657993528,"version":"build-2736575974"},"reference-count":17,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2011,9,5]],"date-time":"2011-09-05T00:00:00Z","timestamp":1315180800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2012,7]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>This study was to develop a real\u2010time florescence quantitative PCR (RT\u2010FQ\u2010PCR) assay to measure virulence\u2010associated DEAD\u2010box RNA helicase (VAD1) mRNA from <jats:italic>Cryptococcus neoformans<\/jats:italic> and evaluate its potential use in diagnosis and follow\u2010up treatment of <jats:italic>C. neoformans<\/jats:italic> meningitis (CNM). <jats:italic>Cryptococcus neoformans<\/jats:italic> was detected using RT\u2010FQ\u2010PCR, ink staining, fungal culturing and <jats:italic>C. neoformans<\/jats:italic> antigen detection in CNM compared with a normal control. VAD1 mRNA was measured in both acute and stable CNM patients. The sensitivity of RT\u2010FQ\u2010PCR (96%) is higher than ink staining (72%) and culture culturing (64%) (<jats:italic>P\u2003<\/jats:italic>&lt;<jats:italic>\u2003<\/jats:italic>0.05, <jats:italic>P\u2003<\/jats:italic>&lt;<jats:italic>\u2003<\/jats:italic>0.05 respectively), but its sensitivity is the same as antigen detection (96%, <jats:italic>P\u2003<\/jats:italic>&gt;<jats:italic>\u2003<\/jats:italic>0.05). The levels of VAD1 mRNA in the acute and stable phase of a <jats:italic>C. neoformans<\/jats:italic> infection are 3.042\u2003\u00b1\u20030.906 and 2.187\u2003\u00b1\u20030.665 respectively (<jats:italic>P\u2003<\/jats:italic>&lt;<jats:italic>\u2003<\/jats:italic>0.01). The levels of VAD1 mRNA are correlated to the numbers of <jats:italic>C. neoformans<\/jats:italic>, intracranial pressure and glucose concentration in cerebrospinal fluid (CSF; <jats:italic>P\u2003<\/jats:italic>&lt;<jats:italic>\u2003<\/jats:italic>0.01, <jats:italic>P\u2003<\/jats:italic>&lt;<jats:italic>\u2003<\/jats:italic>0.01 and <jats:italic>P\u2003<\/jats:italic>&lt;<jats:italic>\u2003<\/jats:italic>0.05 respectively). The levels of expression of VAD1 mRNA in the group of patients who received an AmB\/5\u2010FC\/FZC drug regimen decreased more than in patients taking a 5\u2010FC\/AmB or 5\u2010FC\/FCZ drug combination. Quantitative measurements of VAD1 mRNA are valuable and reliable in diagnosing <jats:italic>C. neoformans<\/jats:italic> infection and evaluating a therapy response.<\/jats:p>","DOI":"10.1111\/j.1439-0507.2011.02100.x","type":"journal-article","created":{"date-parts":[[2011,9,5]],"date-time":"2011-09-05T08:29:50Z","timestamp":1315211390000},"page":"326-332","source":"Crossref","is-referenced-by-count":14,"title":["Comparison of real\u2010time florescence quantitative PCR measurements of VAD1 mRNA with three conventional methods in diagnosis and follow\u2010up treatment of <i>Cryptococcus neoformans<\/i> infection"],"prefix":"10.1111","volume":"55","author":[{"given":"Ou","family":"Qishui","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiang","family":"Ling","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Ni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Bin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Wen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2011,9,5]]},"reference":[{"key":"e_1_2_5_2_2","first-page":"17","article-title":"Disseminated cryptococcosis as inaugural manifestation of AIDS","volume":"94","author":"Fournier Y","year":"2005","journal-title":"Schweiz Rundsch Med Prax"},{"key":"e_1_2_5_3_2","doi-asserted-by":"publisher","DOI":"10.1086\/592690"},{"key":"e_1_2_5_4_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1103773"},{"key":"e_1_2_5_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.8.2860-2865.2002"},{"key":"e_1_2_5_6_2","doi-asserted-by":"publisher","DOI":"10.1590\/S0036-46652004000400006"},{"key":"e_1_2_5_7_2","doi-asserted-by":"crossref","first-page":"632","DOI":"10.1172\/JCI200523048","article-title":"The DEAD\u2010box RNA helicase VAD1 regulates multiple virulence\u2010associated genes in Cryptococcus neoformans","volume":"115","author":"Panepinto J","year":"2005","journal-title":"Clin Invest"},{"key":"e_1_2_5_8_2","doi-asserted-by":"publisher","DOI":"10.1086\/313757"},{"key":"e_1_2_5_9_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI24508"},{"key":"e_1_2_5_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.4.1736-1737.2003"},{"key":"e_1_2_5_11_2","doi-asserted-by":"publisher","DOI":"10.1136\/jnnp.2004.052662"},{"key":"e_1_2_5_12_2","doi-asserted-by":"publisher","DOI":"10.1093\/bmb\/ldh043"},{"key":"e_1_2_5_13_2","first-page":"360","volume-title":"Clinical Fungi","author":"Qing Q","year":"2001"},{"key":"e_1_2_5_14_2","volume-title":"Adams and Victor\u2019s Principles of Neurology","author":"Maurice V","year":"2001"},{"key":"e_1_2_5_15_2","first-page":"203","article-title":"Clinical analysis of 28 cases of cryptococcal meningitis","volume":"28","author":"Chunhua MA","year":"2006","journal-title":"Acta of Third Military Medical University"},{"key":"e_1_2_5_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bmt.1705687"},{"key":"e_1_2_5_17_2","doi-asserted-by":"publisher","DOI":"10.1086\/515110"},{"key":"e_1_2_5_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(04)16301-0"}],"container-title":["Mycoses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1439-0507.2011.02100.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1439-0507.2011.02100.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,14]],"date-time":"2023-10-14T16:44:20Z","timestamp":1697301860000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1439-0507.2011.02100.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,9,5]]},"references-count":17,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2012,7]]}},"alternative-id":["10.1111\/j.1439-0507.2011.02100.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1439-0507.2011.02100.x","archive":["Portico"],"relation":{},"ISSN":["0933-7407","1439-0507"],"issn-type":[{"value":"0933-7407","type":"print"},{"value":"1439-0507","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,9,5]]}}}