{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T02:59:44Z","timestamp":1793501984608,"version":"4.5.0"},"reference-count":37,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2012,2,27]],"date-time":"2012-02-27T00:00:00Z","timestamp":1330300800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2012,5]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p><jats:italic>Cryptococcus<\/jats:italic> isolates from Cuban patients were identified as <jats:italic>C.\u2003neoformans<\/jats:italic> var. <jats:italic>grubii<\/jats:italic>. Although this species has since long been associated with bird droppings, a recent genotyping study provided strong evidence for additional origins of exposure. We sampled different species of trees in Havana, Cuba to identify other potential sources of exposure to this fungus. A total of 662 samples were collected from 331 trees and cacti from Havana, Cuba. Initial selection of the isolates was carried out by conventional techniques. Isolates were further characterised using a combination of AFLP analysis and DNA sequence analysis. Identification by conventional methods yielded 121 <jats:italic>C.\u2003neoformans<\/jats:italic> and 61 <jats:italic>C.\u2003gattii<\/jats:italic> isolates. Molecular analyses showed that none of these isolates was <jats:italic>C.\u2003gattii<\/jats:italic> and only one isolate proved to be <jats:italic>C.\u2003neoformans<\/jats:italic> var. <jats:italic>grubii<\/jats:italic>. A total of 27 different other species were identified. The most prevalent species was <jats:italic>C.\u2003heveanensis<\/jats:italic> (33%). Sixty\u2010five unidentifiable isolates segregated into ten potentially novel species. Conventional cultivation methods have a low specificity for <jats:italic>C.\u2003neoformans<\/jats:italic> complex and molecular analyses need to be applied to confirm identification of isolates from environmental sources. Environmental niches responsible for most of human cryptococcal infections in Cuba remain to be identified.<\/jats:p>","DOI":"10.1111\/j.1439-0507.2012.02168.x","type":"journal-article","created":{"date-parts":[[2012,2,27]],"date-time":"2012-02-27T08:19:45Z","timestamp":1330330785000},"source":"Crossref","is-referenced-by-count":25,"title":["Environmental isolation and characterisation of <i>Cryptococcus<\/i> species from living trees in Havana city, Cuba"],"prefix":"10.1111","volume":"55","author":[{"given":"M. T.","family":"Illnait\u2010Zaragoz\u00ed","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G. 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