{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,12,21]],"date-time":"2024-12-21T21:40:06Z","timestamp":1734817206263,"version":"3.32.0"},"reference-count":26,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2004,4,2]],"date-time":"2004-04-02T00:00:00Z","timestamp":1080864000000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2004,4]]},"abstract":"<jats:title>Contents<\/jats:title><jats:p>An endometritis model was used to investigate the influence of degenerative endometrial changes (endometrosis) on functional parameters of uterine neutrophils in the horse. Six hours after intrauterine application of recombinant human interleukin\u20108 (rhIL\u20108), the uteri of 15 mares were flushed with phosphate\u2010buffered saline. Quantitative and qualitative flow cytometric assays were then made to determine the absolute numbers, viability, phenotype, generation of reactive oxygen species (ROS), and phagocytic activity of immigrated polymorphonuclear neutrophilic granulocytes (PMN). Recombinant hIL\u20108 attracted similarly high numbers of similarly viable PMN into the uteri of mares with or without degenerative endometrial changes. Compared with blood PMN, immigrated uterine neutrophils displayed significantly upregulated expression of CD11a\/CD18 (LFA\u20101) on uterine PMN whereas major histocompatibility complex class I molecules were expressed at lower densities. The ability to phagocytose opsonized streptococci did not differ between uterine and blood PMN. However, uterine PMN displayed a higher capacity to generate ROS. On average, uterine PMN of mares with degenerative endometrial changes showed phenotypical and functional characteristics similar to those of mares with a histologically healthy endometrium. Therefore, degenerative endometrial changes<jats:italic>per se<\/jats:italic>did not reduce the functional capacity of equine uterine neutrophils in mares.<\/jats:p>","DOI":"10.1111\/j.1439-0531.2004.00486.x","type":"journal-article","created":{"date-parts":[[2004,12,28]],"date-time":"2004-12-28T17:40:51Z","timestamp":1104255651000},"page":"94-98","source":"Crossref","is-referenced-by-count":3,"title":["Degenerative Endometrial Changes do not Change the Functional Capacity of Immigrating Uterine Neutrophils in Mares"],"prefix":"10.1111","volume":"39","author":[{"given":"H","family":"Zerbe","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F","family":"Engelke","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E","family":"Klug","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H\u2010A","family":"Schoon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W","family":"Leibold","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,4,2]]},"reference":[{"key":"e_1_2_11_2_1","first-page":"311","article-title":"Effects of susceptibility to endometritis and stage of cycle on phagocytic activity of uterine\u2010derived neutrophils","volume":"35","author":"Asbury AC","year":"1987","journal-title":"J 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