{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T02:45:35Z","timestamp":1781750735465,"version":"3.54.5"},"reference-count":19,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2004,9,13]],"date-time":"2004-09-13T00:00:00Z","timestamp":1095033600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2004,10]]},"abstract":"<jats:title>Contents<\/jats:title><jats:p>Traditional treatments of two prostaglandin F2 alpha (PGF2<jats:italic>\u03b1<\/jats:italic>) doses at 10\u2010day intervals or more did not result in acceptable pregnancy rates in timed artificial insemination (TAI) programmes in ewes. An explanation might be the undefined time\u2010period of the onset of oestrus and ovulation after the treatment. Recently a consistent interval to oestrus and ovulation was obtained by giving PGF2<jats:italic>\u03b1<\/jats:italic> at day 3 post\u2010ovulation, i.e. when the largest follicle of the first follicular wave of the cycle was still growing. This can be achieved when a second dose of PGF2<jats:italic>\u03b1<\/jats:italic> is given 7\u2003days after a first dose. In this work, we evaluated the synchronization of oestrus and determined which of three different moments of TAI was the most successful using a PGF2<jats:italic>\u03b1<\/jats:italic> (PG\u20107d) protocol in a large flock. A total of 436 nulliparous and multiparous ewes were treated with two doses of a PGF2<jats:italic>\u03b1<\/jats:italic> analogue (delprostenate 160\u2003<jats:italic>\u03bc<\/jats:italic>g, i.m.) separated by 7\u2003days. Onset of oestrus was recorded twice a day and a single cervical TAI with fresh undiluted semen was performed either at 42\u2003h (n\u2003=\u2003152), 48\u2003h (n\u2003=\u2003120), or 54\u2003h (n\u2003=\u2003164), after the second PGF2<jats:italic>\u03b1<\/jats:italic> dose without taking into account the oestrous response. Pregnancy rate was determined by transrectal ultrasonography 30\u2003days after insemination. Onset of oestrus was detected in 308 of 328 and 89 of 108 multiparous and nulliparous ewes, respectively (p\u2003&lt;\u20030.001), within 72\u2003h after treatment. The distribution of the onset of oestrus did not differ between multiparous and nulliparous ewes and the highest proportion of ewes in oestrus was detected between 25 to 48\u2003h (313\/397) from the second PGF2<jats:italic>\u03b1<\/jats:italic> dose. The pregnancy rate in ewes inseminated at 42\u2003h tended to be higher than those inseminated at 48\u2003h (p\u2003=\u20030.09) and was higher than those inseminated at 54\u2003h (p\u2003&lt;\u20030.05) (56\/152, 31\/120, 37\/164; respectively). Therefore, the use of the PG\u20107d protocol resulted in a very high synchronization of oestrus with the highest concentration (around 80%) between 25 to 48\u2003h from the end of treatment. The best pregnancy rate (37%) was obtained after a single cervical TAI with fresh semen at 42\u2003h.<\/jats:p>","DOI":"10.1111\/j.1439-0531.2004.00527.x","type":"journal-article","created":{"date-parts":[[2004,9,13]],"date-time":"2004-09-13T13:13:26Z","timestamp":1095081206000},"page":"352-355","source":"Crossref","is-referenced-by-count":45,"title":["Prostaglandin F2\u03b1 Treatment Associated with Timed Artificial Insemination in Ewes"],"prefix":"10.1111","volume":"39","author":[{"given":"A","family":"Menchaca","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V","family":"Miller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J","family":"Gil","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A","family":"Pinczak","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Laca","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E","family":"Rubianes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2004,9,13]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1051\/rnd:19820305"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.1150111"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0021859600060123"},{"key":"e_1_2_6_5_1","volume-title":"Manual pr\u00e1ctico de reproducci\u00f3n e inseminaci\u00f3n artificial en ovinos","author":"Dur\u00e1n del Campo A","year":"1993"},{"key":"e_1_2_6_6_1","volume-title":"Salamon's Artificial Insemination of Sheep and Goats","author":"Evans G","year":"1990"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0093-691X(94)00074-5"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-08-024409-9.50019-7"},{"issue":"1","key":"e_1_2_6_9_1","first-page":"282","article-title":"Fertility and prolificacy of confined ewes treated with prostaglandin F2\u03b1 and bred by AI","volume":"51","author":"Hackett AJ","year":"1980","journal-title":"J Anim Sci"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0093-691X(81)90063-7"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.2527\/jas1973.3761380x"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-4320(91)90062-5"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(98)00148-4"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0093-691X(81)90057-1"},{"key":"e_1_2_6_15_1","unstructured":"MenchacaA GilJ OliveraJ RubianesJ 2003:Efecto de la condici\u00f3n corporal previo al servicio sobre la fertilidad y fecundidad en ovejas inseminadas artificialmente(Effect of body condition score at time of service on fertility and fecundity in artificially inseminated ewes. Proceedings of the V International Symposium on Animal Reproduction C\u00f3rdoba Argentina pp.404(abs.)."},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4320(03)00046-0"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0021859600024874"},{"key":"e_1_2_6_18_1","volume-title":"SAS User's Guide: Statistics","author":"SAS Institute Inc.","year":"1989"},{"key":"e_1_2_6_19_1","first-page":"1351","article-title":"Origin of preovulatory follicles after induced luteolysis during the early luteal phase in ewes","volume":"51","author":"Vi\u00f1oles C","year":"1998","journal-title":"Can J Anim Sci"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-4320(92)90097-W"}],"container-title":["Reproduction in Domestic Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1439-0531.2004.00527.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1439-0531.2004.00527.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,9]],"date-time":"2023-10-09T23:29:05Z","timestamp":1696894145000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1439-0531.2004.00527.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,9,13]]},"references-count":19,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2004,10]]}},"alternative-id":["10.1111\/j.1439-0531.2004.00527.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1439-0531.2004.00527.x","archive":["Portico"],"relation":{},"ISSN":["0936-6768","1439-0531"],"issn-type":[{"value":"0936-6768","type":"print"},{"value":"1439-0531","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,9,13]]}}}