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Membrane vesicles were present in cellular extracts from reproductive organs and accessory sex glands of six rams, and in the seminal plasma of a further eight rams. When four of the latter rams were subjected to vasectomy, to isolate ejaculate contents to only the secretions of the accessory sex glands, the vesicles were largely eliminated from their ejaculates, while vesicles were still present in the ejaculates of the four control rams. The constituents of the cytoplasmic droplets and membrane vesicles derived from the seminal plasma were compared by transmission electron microscopy (TEM). Vesicles present in the cytoplasmic droplets were similar in morphology but smaller on average than those in the seminal plasma. It was concluded that the membrane vesicles in ram seminal plasma originate from either the cytoplasmic droplets, or a combination of vesicles from the droplets and the epididymis.<\/jats:p>","DOI":"10.1111\/j.1439-0531.2006.00642.x","type":"journal-article","created":{"date-parts":[[2006,2,27]],"date-time":"2006-02-27T17:51:04Z","timestamp":1141062664000},"page":"98-105","source":"Crossref","is-referenced-by-count":5,"title":["The Origin of Membrane Vesicles in Ram Seminal Plasma"],"prefix":"10.1111","volume":"41","author":[{"given":"R","family":"El\u2010Hajj Ghaoui","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"PC","family":"Thomson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G","family":"Evans","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"WMC","family":"Maxwell","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,2,27]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0790409"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1939-4640.1988.tb01056.x"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s002329900160"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod59.2.309"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00381652"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.3109\/01485018208990233"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.3109\/03009738309178440"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0531.2004.00499.x"},{"key":"e_1_2_6_10_1","volume-title":"Salamon's Artificial Insemination of Sheep and Goats","author":"Evans G","year":"1987"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.humrep.a138735"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M107005200"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0040-8166(84)90063-6"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)52451-6"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(78)90586-9"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.1140237"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.167.9.5061"},{"key":"e_1_2_6_18_1","first-page":"221","article-title":"Post\u2010mortem changes at electron microscope level in the type II cells of the lung","volume":"55","author":"Pattle RE","year":"1974","journal-title":"Br J Exp Pathol"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(79)90222-7"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0854.2000.010901.x"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4157(85)90008-5"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0272.1978.tb03030.x"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0272.1978.tb03064.x"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1939-4640.2003.tb02653.x"},{"key":"e_1_2_6_25_1","first-page":"873","article-title":"Programmed cell death","volume":"65","author":"Samuilov V","year":"2000","journal-title":"Biochemistry"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00255932"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.3109\/00365598209179734"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/8.11.984"}],"container-title":["Reproduction in Domestic Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1439-0531.2006.00642.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1439-0531.2006.00642.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,10]],"date-time":"2023-10-10T06:13:51Z","timestamp":1696918431000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1439-0531.2006.00642.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,2,27]]},"references-count":27,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2006,4]]}},"alternative-id":["10.1111\/j.1439-0531.2006.00642.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1439-0531.2006.00642.x","archive":["Portico"],"relation":{},"ISSN":["0936-6768","1439-0531"],"issn-type":[{"value":"0936-6768","type":"print"},{"value":"1439-0531","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,2,27]]}}}