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Semen was collected using a computer\u2010controlled automated phantom that separates the ejaculates into five successive cups. Samples containing seminal plasma and skim milk extender were compared with samples stored in skim milk extender after the removal of seminal plasma by centrifugation. Fractionated ejaculates were stored cooled for 24\u2003h after dilution with extender (Expt 1) or frozen in liquid nitrogen (Expt 2). In Expt 1, cup 1 was pre\u2010sperm fluid, cups 2 and 3 sperm\u2010rich fractions, and cup 4 sperm\u2010poor fractions. In Expt 2, cups 1 and 2 were sperm\u2010rich fractions, and cups 3 and 4 sperm\u2010poor fractions. One sample (WE) represented the whole ejaculate in both experiments. Motility parameters were determined with a Hamilton\u2010Thorn Motility Analyzer, and plasma membrane integrity was assessed using carboxyfluorescein diacetate and propidium iodide staining and fluorescence microscopy. The removal of seminal plasma lowered motility values, but not plasma membrane integrity, in both experiments. No significant differences between cups were observed after cooled storage. The cups differed significantly in most post\u2010thaw motility parameters, and the sperm\u2010rich fraction showed higher post\u2010thaw motility than the whole ejaculate.<\/jats:p>","DOI":"10.1111\/j.1439-0531.2006.00647.x","type":"journal-article","created":{"date-parts":[[2006,1,12]],"date-time":"2006-01-12T15:54:11Z","timestamp":1137081251000},"page":"33-38","source":"Crossref","is-referenced-by-count":29,"title":["Motility and Plasma Membrane Integrity of Spermatozoa in Fractionated Stallion Ejaculates after Storage"],"prefix":"10.1111","volume":"41","author":[{"given":"AM","family":"Kareskoski","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T","family":"Reilas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Andersson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T","family":"Katila","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,1,12]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-4320(93)90095-9"},{"key":"e_1_2_5_3_1","first-page":"113","article-title":"Proteins in stallion seminal plasma","volume":"35","author":"Amann RP","year":"1987","journal-title":"J Reprod Fertil Suppl"},{"key":"e_1_2_5_4_1","first-page":"34","article-title":"Effects of seminal plasma on stallion semen quality after cryopreservation","volume":"15","author":"Aurich JE","year":"1998","journal-title":"J Reprod Fertil Abstr Ser"},{"key":"e_1_2_5_5_1","first-page":"167","article-title":"Decapacitation factors in semen","volume":"1","author":"Begley AJ","year":"1982","journal-title":"Clin Reprod Fertil"},{"key":"e_1_2_5_6_1","first-page":"253","volume-title":"Manual of Equine Reproduction","author":"Blanchard TL","year":"2003"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0093-691X(94)90497-7"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0749-0739(17)30476-5"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(00)00331-9"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.2527\/jas1976.433633x"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(01)00540-4"},{"key":"e_1_2_5_12_1","doi-asserted-by":"crossref","first-page":"626","DOI":"10.2527\/jas1976.433626x","article-title":"Reproductive physiology of the stallion. 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