{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T16:44:54Z","timestamp":1762015494633},"reference-count":24,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2006,3,8]],"date-time":"2006-03-08T00:00:00Z","timestamp":1141776000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2006,6]]},"abstract":"<jats:title>Contents<\/jats:title><jats:p>A Burdizzo castrator was evaluated for the neutering of dogs. Histological and morphological changes of spermatic cells and peripheral serum testosterone after challenge with a GnRH\u2010analogue (gonadorelin) were assessed. There was a control group (G1), a surgically castrated group (G2) and a Burdizzo group (G3) divided in two, G3a receiving two crunches in each spermatic cord and G3b receiving one crunch in each spermatic cord. Sixteen days after application of the Burdizzo blood samples were taken from the dogs at 30\u2003min interval during 2\u2003h; after the second sample the dogs were treated with 1\u2003<jats:italic>\u03bc<\/jats:italic>g\/kg body weight of gonadorelin i.v. The same protocol of gonadorelin challenge was performed in G1 and G2 dogs. The G2 dogs were surgically castrated after the second blood sample, before the gonadorelin treatment, and the G1 dogs after the last blood sample. The excised gonads were examined histologically, and sperm smears were prepared from the caudae epididymidis. The testes and plexus pampiniformis of the G1 and G2 dogs had a normal histological appearance, and they had morphologically normal epididymal sperm cells. In all G3 dogs, there was an acute fibrosis with an inflammatory reaction in the plexus pampiniformis. The testes from the G3a dogs showed diffuse areas of infarction and degeneration of the parenchyma. Similar but less diffuse lesions were seen in group 3b dogs. The deferent ducts from all G3 dogs showed vasitis and\/or sperm granulomas. Azoospermia or sperm malformations were observed in the epididymal smears from the G3 dogs. Testosterone concentration in the G1 dogs increased after gonadorelin application (p\u2003&lt;\u20030.0001). The G2 dogs had basal testosterone levels after castration (p\u2003&lt;\u20030.001) and did not respond to gonadorelin. Groups 3a and b showed a slight but non\u2010significant increase in testosterone concentration after gonadorelin challenge, supposedly due to the reduction of testicular blood flow and loss of testicular interstitial tissue.<\/jats:p>","DOI":"10.1111\/j.1439-0531.2006.00668.x","type":"journal-article","created":{"date-parts":[[2006,3,8]],"date-time":"2006-03-08T16:03:28Z","timestamp":1141833808000},"page":"227-232","source":"Crossref","is-referenced-by-count":10,"title":["Evaluation of a Burdizzo Castrator for Neutering of 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