{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T16:11:29Z","timestamp":1759939889628},"reference-count":43,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2006,8,10]],"date-time":"2006-08-10T00:00:00Z","timestamp":1155168000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2006,10]]},"abstract":"<jats:title>Contents<\/jats:title><jats:p>The aim of this study was to investigate the influence of pre\u2010 and post\u2010ovulatory insemination and early pregnancy on the distribution of immune cells in the oviduct. Eighteen sows were pre\u2010ovulatory and sixteen sows were post\u2010ovulatory inseminated and slaughtered at different times, 5\u20136\u2003h after insemination, 20\u201325\u2003h and approximately 70\u2003h after ovulation, day 11 and day 19. Immediately after slaughter, oviductal samples of three different segments (isthmus, ampulla and infundibulum) were fixed, embedded in plastic resin and stained with toluidine blue or cryofixed and stored in a freezer at \u221270\u00b0C until analysed by immunohistochemistry (pre\u2010ovulatory inseminated sows) with an avidin\u2013biotin peroxidase method. Quantitative and qualitative examinations of oviductal epithelium and subepithelial connective tissue were performed by light microscopy. After pre\u2010 or post\u2010ovulatory insemination, neutrophils were not observed in the oviductal epithelium from any of the segments or groups. The numbers of intraepithelial lymphocytes of all sows as well as CD2\u2010 and CD3\u2010positive cells of the pre\u2010ovulatory inseminated sows were higher in the infundibulum than in the other segments (p\u2003\u2264\u20030.001). In the subepithelial connective tissue of the pre\u2010ovulatory inseminated sows, significantly higher numbers of lymphocytes (p\u2003\u2264\u20030.001) and plasma cells (p\u2003\u2264\u20030.001) were found in infundibulum than in isthmus. Neutrophils were found mainly in infundibulum, the number approximately 40\u2003h after pre\u2010ovulatory insemination was significantly higher (p\u2003\u2264\u20030.05) than in the other groups and segments. Significantly higher numbers of CD2 than CD3\u2010positive cells were found for all groups and segments. In the subepithelial connective tissue of post\u2010ovulatory inseminated sows, the numbers of lymphocytes was higher (p\u2003\u2264\u20030.001) at day 19 than up to 50\u2003h after insemination and lower (p\u2003\u2264\u20030.001) in isthmus than in ampulla and infundibulum. Neutrophils were found in infundibulum in almost all groups and the number was significantly higher (p\u2003\u2264\u20030.05) in the infundibulum up to 50\u2003h after insemination than in other segments. In the oviductal epithelium, no influence of insemination was found on the presence of phagocytes, i.e. neutrophils and macrophages, but on lymphocytes. In the infundibular connective tissue, pre\u2010ovulatory insemination had an effect on neutrophil distribution, indicating an active immune response to insemination in the upper segment. Post\u2010ovulatory insemination changed the oviductal immune cell pattern.<\/jats:p>","DOI":"10.1111\/j.1439-0531.2006.00695.x","type":"journal-article","created":{"date-parts":[[2006,8,10]],"date-time":"2006-08-10T09:59:37Z","timestamp":1155203977000},"page":"455-466","source":"Crossref","is-referenced-by-count":10,"title":["The Influence of Pre\u2010 and Post\u2010ovulatory Insemination and Early Pregnancy on the Infiltration by Cells of the Immune System in the Sow 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