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Selective inhibitor of NOS, <jats:italic>N<\/jats:italic>\u2010<jats:italic>\u03c9<\/jats:italic>\u2010nitro\u2010<jats:sc>l<\/jats:sc>\u2010arginine methyl ester (<jats:sc>l<\/jats:sc>\u2010NAME) was applied at different doses (0, 0.1, 1 and 10\u2003m<jats:sc>m<\/jats:sc>) in maturation (experiment 1A), culture medium (experiment 1B) and in both maturation and culture media (experiment 1C). No significant differences were observed in cleavage and blastocyst rates when oocytes were matured in the presence of <jats:sc>l<\/jats:sc>\u2010NAME as long as the inhibitor was omitted during fertilization and culture. However, significantly lower blastocyst rates were observed when <jats:sc>l<\/jats:sc>\u2010NAME was present at higher level (10\u2003m<jats:sc>m<\/jats:sc>) in culture medium alone and in both maturation and culture media. In experiment 2, mRNA isolated from triplicate pools of oocytes and embryos (n\u2003=\u200315\u201320) was subjected to quantitative real time reverse transcription polymerase chain reaction to investigate the expression of eNOS, iNOS and nNOS mRNA in normal IVP bovine oocytes and embryos. While eNOS and iNOS transcripts were detected at higher level in oocytes (immature and mature), two\u2010cell and four\u2010cell stage embryos, the nNOS was detected only in immature oocyte, two\u2010cell and morula stages. In experiment 3, eNOS and iNOS protein expression analysis was performed in IVP oocytes and embryos and both proteins were detected in the cytoplasm and the nuclei (weak) of oocytes and embryos. These data provide the first evidence for the role of NO production and the presence of mRNA and protein products of NOS isoforms during bovine embryogenesis.<\/jats:p>","DOI":"10.1111\/j.1439-0531.2006.00701.x","type":"journal-article","created":{"date-parts":[[2006,8,22]],"date-time":"2006-08-22T08:20:45Z","timestamp":1156234845000},"page":"501-509","source":"Crossref","is-referenced-by-count":33,"title":["The Effect of Nitric Oxide Inhibition and Temporal Expression Patterns of the mRNA and Protein Products of Nitric Oxide Synthase Genes During <i>In Vitro<\/i> Development of Bovine Pre\u2010implantation 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