{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T04:33:16Z","timestamp":1767846796860,"version":"3.49.0"},"reference-count":21,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2007,1,23]],"date-time":"2007-01-23T00:00:00Z","timestamp":1169510400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2007,4]]},"abstract":"<jats:title>Contents<\/jats:title><jats:p>Frozen\u2010thawed (FT) boar sperm have a reduced fertile life, due in part to a capacitation\u2010like status induced by cooling. Reversal of this cryocapacitation <jats:italic>in vitro<\/jats:italic> by exposure to boar seminal plasma (SP) has been demonstrated. The objective of these studies was to determine the effect of SP on the ability of FT sperm to create an oviductal sperm reservoir following artificial insemination (AI). In Experiment one, 35 pre\u2010pubertal gilts were injected (IM) with 400\u2003IU eCG plus 200\u2003IU hCG to induce oestrus. At detection of oestrus, gilts were inseminated with 3\u2003\u00d7\u200310<jats:sup>9<\/jats:sup> live sperm, either fresh (FS; n\u2003= 13), FT (n\u2003=\u200310), or FT supplemented with 10% v\/v SP (n\u2003= 12). Gilts were killed 8\u2003h later, their reproductive tracts recovered and the uterotubal junctions (UTJs) flushed to recover sperm. Fewer (p\u2003&lt;\u20030.01) sperm were recovered following FT, compared to FS, inseminations, and there was no evident effect of SP. In Experiment two, 30 pre\u2010pubertal gilts received IM injections of 1000\u2003IU eCG followed by 5\u2003mg pLH 80\u2003h later to control time of ovulation. Gilts were inseminated with 3\u2003\u00d7\u200310<jats:sup>9<\/jats:sup> live FS sperm (n\u2003=\u20036), FT sperm (n\u2003=\u200315) or FT sperm plus 10% SP (n\u2003=\u20039) at 12\u2003h before ovulation and then sacrificed 8\u2003h later. The UTJs were dissected and flushed for sperm recovery. Fewest (p\u2003&lt;\u20030.001) sperm were recovered following FT insemination and there was no evident effect of SP. These data demonstrate that the size of the sperm reservoir is markedly reduced in gilts inseminated with FT sperm. However, the lack of effect of SP suggested that either it did not reverse cryocapacitation or that such a reversal does not impact the in vivo ability to create a sperm reservoir.<\/jats:p>","DOI":"10.1111\/j.1439-0531.2006.00744.x","type":"journal-article","created":{"date-parts":[[2007,1,23]],"date-time":"2007-01-23T11:45:07Z","timestamp":1169552707000},"page":"149-152","source":"Crossref","is-referenced-by-count":19,"title":["Effect of Sperm Cryopreservation and Supplementing Semen Doses with Seminal Plasma on the Establishment of a Sperm Reservoir in Gilts"],"prefix":"10.1111","volume":"42","author":[{"given":"M","family":"Abad","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"DJ","family":"Sprecher","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P","family":"Ross","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"RM","family":"Friendship","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"RN","family":"Kirkwood","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,1,30]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.4141\/A03-024"},{"key":"e_1_2_6_3_1","unstructured":"BuhrM 2001:Emerging Tools in Artificial Insemination. 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