{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,8]],"date-time":"2026-02-08T02:55:03Z","timestamp":1770519303054,"version":"3.49.0"},"reference-count":28,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2007,2,5]],"date-time":"2007-02-05T00:00:00Z","timestamp":1170633600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2007,6]]},"abstract":"<jats:title>Contents<\/jats:title><jats:p>This study describes ovine pregnancy\u2010associated glycoprotein (ovPAG) concentrations in 20 Lacaune sheep during early pregnancy. Measurements were performed by using semi\u2010purified ovPAG as standard, tracer and immunogens for antibody production in rabbits. Antisera R780 (against ovPAG<jats:sub>57+59kDa<\/jats:sub>) and R805 (against ovPAG5<jats:sub>58+61kDa<\/jats:sub>) were used respectively in RIA\u2010780 and RIA\u2010805. Blood samples were collected at days 0, 18, 20, 22 and 25 after artificial insemination. From day 18 after breeding onward, the mean ovPAG concentration was significantly higher (p\u2003&lt;\u20030.001) in plasma samples from pregnant ewes (n\u2003=\u200317) than in non\u2010pregnant ones (n\u2003=\u20033). The specific activity of the tracer was 11\u2003760\u2003Ci\/mmol in RIA\u2010780 and 14\u2003900\u2003Ci\/mmol in RIA\u2010805. The minimal detection limits for RIA\u2010780 and RIA\u2010805 were 0.2\u2003ng\/ml and 0.3\u2003ng\/ml, respectively. The intra\u2010assay CV of samples with low (1.0\u2003ng\/ml), medium (2.5\u2003ng\/ml) and high (4.0\u2003ng\/ml) PAG concentrations were 3%, 6% and 9% for RIA\u2010780 and 8%, 9% and 5% for RIA\u2010805. The inter\u2010assay CV in the same samples were 13%, 12% and 7% for RIA\u2010780 and 13%, 11% and 5% for RIA\u2010805. The recovery was higher than 95% in both assays. No cross\u2010reaction was observed with members of aspartic proteinase family as well as with other tested proteins. In both RIA\u2010780 and RIA\u2010805, inhibition of the binding of the tracer by antisera was parallel between standard curve and serial dilutions of pregnant ewe samples. In conclusion, the two homologous RIA systems are suitable for early quantification of ovPAG concentrations in ewe plasma samples from day 18 after breeding.<\/jats:p>","DOI":"10.1111\/j.1439-0531.2006.00761.x","type":"journal-article","created":{"date-parts":[[2007,2,5]],"date-time":"2007-02-05T19:25:05Z","timestamp":1170703505000},"page":"257-262","source":"Crossref","is-referenced-by-count":14,"title":["Measurement of Ovine Pregnancy\u2010Associated Glycoprotein (PAG) During Early Pregnancy in Lacaune Sheep"],"prefix":"10.1111","volume":"42","author":[{"given":"B","family":"El Amiri","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A","family":"Karen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J","family":"Sulon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N","family":"Melo de Sousa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"AV","family":"Alvarez\u2010Oxiley","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y","family":"Cogni\u00e9","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"O","family":"Szenci","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"JF","family":"Beckers","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,2,5]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod26.5.925"},{"key":"e_1_2_6_3_1","doi-asserted-by":"crossref","first-page":"1245","DOI":"10.1093\/clinchem\/33.7.1245","article-title":"A linear method for determining specific activity of tracers in radioimmunoassay","volume":"33","author":"Chiang CS","year":"1987","journal-title":"Clin Chem"},{"key":"e_1_2_6_4_1","doi-asserted-by":"crossref","first-page":"79","DOI":"10.20870\/productions-animales.2003.16.2.3648","article-title":"Diagnotic et suivi de gestation chez la brebis: r\u00e9alit\u00e9s et perspectives","volume":"16","author":"El Amiri B","year":"2003","journal-title":"Prod Anim INRA"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.10246"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1051\/rnd:2004025"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0143-4004(86)80162-X"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(99)00165-X"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod62.6.1624"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj0890114"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1051\/rnd:2004005"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(02)01289-X"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1439-0531.2002.t01-1-00366.x"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0093-691X(94)90691-B"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0921-4488(96)00922-4"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0093-691X(88)90227-0"},{"key":"e_1_2_6_17_1","volume-title":"SAS User's guide","author":"SAS\/STAT Institute","year":"2001"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod35.4.936"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/19.2.146"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(98)00115-0"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1210\/jcem-33-6-988"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2004.08.009"},{"key":"e_1_2_6_23_1","first-page":"119","article-title":"A new test for early pregnancy diagnosis in sheep: determination of ovine pregnancy\u2010associated glycoprotein (ovPAG) concentration by means of a homologous radioimmunoassay","volume":"73","author":"Verberckmoes S","year":"2004","journal-title":"Flemish Vet J"},{"key":"e_1_2_6_24_1","doi-asserted-by":"crossref","first-page":"960","DOI":"10.2527\/1995.734960x","article-title":"Detection of fetal twins in sheep using a radioimmunoassay for pregnancy\u2010specific protein B","volume":"73","author":"Willard JM","year":"1995","journal-title":"J Anim Sci"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.88.22.10247"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod57.6.1384"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/0093-691X(90)90790-Z"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod46.1.83"},{"key":"e_1_2_6_29_1","first-page":"177","article-title":"Isolement et caract\u00e9risation partielle d'une glycoprot\u00e9ine associ\u00e9e \u00e0 la gestation chez la brebis","volume":"139","author":"Zoli AP","year":"1995","journal-title":"Ann Med Vet"}],"container-title":["Reproduction in Domestic Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1439-0531.2006.00761.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1439-0531.2006.00761.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,10]],"date-time":"2023-10-10T14:53:55Z","timestamp":1696949635000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1439-0531.2006.00761.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,2,5]]},"references-count":28,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2007,6]]}},"alternative-id":["10.1111\/j.1439-0531.2006.00761.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1439-0531.2006.00761.x","archive":["Portico"],"relation":{},"ISSN":["0936-6768","1439-0531"],"issn-type":[{"value":"0936-6768","type":"print"},{"value":"1439-0531","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,2,5]]}}}