{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,20]],"date-time":"2026-06-20T15:02:35Z","timestamp":1781967755944,"version":"3.54.5"},"reference-count":16,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2007,6,5]],"date-time":"2007-06-05T00:00:00Z","timestamp":1181001600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2007,10]]},"abstract":"<jats:title>Contents<\/jats:title><jats:p>Motility characteristics (assessed subjectively and with computer\u2010assisted semen analysis) and membrane status (after staining with chlortetracycline) of washed and non\u2010washed frozen\u2013thawed ram spermatozoa were evaluated after incubation in buffer and buffer containing autologous whole seminal plasma or one of its two fractions: the pellet of membrane vesicles obtained by ultracentrifugation (and used at three times normal protein concentration) or the vesicle\u2010free supernatant fraction. Whole seminal plasma and supernatant, but not membrane vesicles, improved the motility characteristics of spermatozoa after 3 and 6\u2003h of post\u2010thaw incubation compared with the control buffer. Resuspension and incubation with whole seminal plasma, supernatant or membrane vesicles lowered the proportion of acrosome\u2010reacted frozen\u2013thawed spermatozoa compared with the control buffer. Unwashed frozen\u2013thawed semen from three rams, incubated with autologous whole seminal plasma or its fractions and inseminated using cervical or intrauterine artificial insemination, had no effect on pregnancy rates of ewes in synchronized oestrus. However, fertility was higher after laparoscopic than cervical insemination (44.9 vs 12.3%, p\u2003&lt;\u20030.001). In conclusion, resuspension and incubation of frozen\u2013thawed ram spermatozoa in autologous whole seminal plasma or its vesicle\u2010free supernatant fraction improved their motility characteristics and, with membrane vesicles, membrane status, but these benefits were not reflected in improved fertility after cervical or intrauterine insemination.<\/jats:p>","DOI":"10.1111\/j.1439-0531.2006.00819.x","type":"journal-article","created":{"date-parts":[[2007,6,5]],"date-time":"2007-06-05T09:41:47Z","timestamp":1181036507000},"page":"541-549","source":"Crossref","is-referenced-by-count":28,"title":["Autologous Whole Ram Seminal Plasma and its Vesicle\u2010free Fraction Improve Motility Characteristics and Membrane Status but not <i>In Vivo<\/i> Fertility of Frozen\u2013Thawed Ram Spermatozoa"],"prefix":"10.1111","volume":"42","author":[{"given":"R","family":"El\u2010Hajj Ghaoui","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"PC","family":"Thomson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T","family":"Leahy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G","family":"Evans","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"WMC","family":"Maxwell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2007,6,5]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2736(83)90505-9"},{"key":"e_1_2_6_3_1","first-page":"400","volume-title":"Sheep Breeding","author":"Edey TN","year":"1976"},{"key":"e_1_2_6_4_1","article-title":"Effect of seminal plasma fractions from entire and vasectomised rams on the motility characteristics, membrane status and in vitro fertility of ram spermatozoa","author":"El\u2010Hajj Ghaoui R","year":"2007","journal-title":"J Androl"},{"key":"e_1_2_6_5_1","volume-title":"Salamon's Artificial Insemination of Sheep and Goats","author":"Evans G","year":"1987"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(02)00652-0"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(02)01178-0"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1071\/R96046"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(98)00019-3"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1071\/RD9930057"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1071\/RD99046"},{"key":"e_1_2_6_12_1","article-title":"Seminal plasma effects on sperm handling and female fertility","author":"Maxwell WMC","year":"2007","journal-title":"Reproduction"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2005.04.009"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1677\/joe.0.0490407"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-4320(94)01327-I"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1939-4640.1999.tb02519.x"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1071\/RD9950871"}],"container-title":["Reproduction in Domestic Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1439-0531.2006.00819.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1439-0531.2006.00819.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,14]],"date-time":"2023-10-14T16:54:10Z","timestamp":1697302450000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1439-0531.2006.00819.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,6,5]]},"references-count":16,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2007,10]]}},"alternative-id":["10.1111\/j.1439-0531.2006.00819.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1439-0531.2006.00819.x","archive":["Portico"],"relation":{},"ISSN":["0936-6768","1439-0531"],"issn-type":[{"value":"0936-6768","type":"print"},{"value":"1439-0531","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,6,5]]}}}