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Ewes (n\u2003=\u2003360) were inseminated with 1\u2003\u00d7\u200310<jats:sup>6<\/jats:sup>, 5\u2003\u00d7\u200310<jats:sup>6<\/jats:sup> or 15\u2003\u00d7\u200310<jats:sup>6<\/jats:sup> motile sorted frozen\u2010thawed (S<jats:sub>1<\/jats:sub>, S<jats:sub>5<\/jats:sub>, or S<jats:sub>15<\/jats:sub> respectively) or non\u2010sorted frozen\u2010thawed (C<jats:sub>1<\/jats:sub>, C<jats:sub>5<\/jats:sub> or C<jats:sub>15<\/jats:sub> respectively) spermatozoa from three rams. An additional group of ewes were inseminated with 50\u2003\u00d7\u200310<jats:sup>6<\/jats:sup> motile non\u2010sorted frozen\u2010thawed spermatozoa (C<jats:sub>50<\/jats:sub>) to provide a commercial dose control. The percentage of ewes lambing after insemination was similar for C<jats:sub>50<\/jats:sub> (24\/38, 63.2%), C<jats:sub>15<\/jats:sub> (37\/54, 68.5%), S<jats:sub>15<\/jats:sub> (38\/57, 66.7%), S<jats:sub>5<\/jats:sub> (37\/56, 66.1%) and S<jats:sub>1<\/jats:sub> (32\/52, 61.5%) groups (p\u2003&gt;\u20030.05), but lower for C<jats:sub>5<\/jats:sub> (19\/48, 39.6%) and C<jats:sub>1<\/jats:sub> (19\/55, 34.5%) treatments (p\u2003&lt;\u20030.05). This study demonstrates sorted ram spermatozoa are equally fertile to non\u2010sorted spermatozoa even when inseminated at 2% of the dose. Furthermore, at very low artificial insemination doses (1 or 5\u2003million motile) the fertility of sorted ram spermatozoa is superior to non\u2010sorted spermatozoa inseminated in equal numbers. These results have significance for the future commercialization of sex\u2010preselection technology in sheep as a reduction in the minimum effective sperm number will allow a corresponding decrease in the associated cost per dose.<\/jats:p>","DOI":"10.1111\/j.1439-0531.2006.00837.x","type":"journal-article","created":{"date-parts":[[2007,10,30]],"date-time":"2007-10-30T01:58:28Z","timestamp":1193709508000},"page":"648-653","source":"Crossref","is-referenced-by-count":35,"title":["Successful Low Dose Insemination of Flow Cytometrically Sorted Ram Spermatozoa in Sheep"],"prefix":"10.1111","volume":"42","author":[{"given":"SP","family":"De 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