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Co\u2010incubation of pbUEC and polymorphonuclear (PMN) granulocytes derived from peripheral bovine blood samples, was performed before testing the cell culture supernatant for production of interleukin\u20108 (IL\u20108) via ELISA. Cytokine secretion was only detectable after co\u2010incubation of pbUEC with PMN, whereas neither pbUEC nor PMN alone generated IL\u20108 in relevant chemo attractive doses. Another objective was to examine the influence of bovine seminal plasma (SP) and vesicular gland fluid (VGF) on various functional parameters of PMN including cell viability, production of reactive oxygen species and chemotaxis. Analysis of these effects was conducted by flow cytometry. Viability of PMN was determined by staining the cells with propidium iodide. Seminal plasma was added to suspensions of PMN in increasing increments and resulted in a significant increase of cell membrane damaged PMN when using SP concentrations above 0.2%. The reactive oxygen species production of PMN suspensions, stimulated with phorbol\u201012\u2010myristate\u201013\u2010acetate, was significantly decreased by 30% up to 90% when adding 0.06\u201330\u2030 of either SP or VGF. The PMN transmigration induced by IL\u20108 was diminished by 50% when 0.4\u2030 of either SP or VGF were added. The results of this study indicate a potential regulatory impact of SP and VGF on inflammatory processes in the bovine uterus.<\/jats:p>","DOI":"10.1111\/j.1439-0531.2011.01792.x","type":"journal-article","created":{"date-parts":[[2011,4,30]],"date-time":"2011-04-30T07:26:41Z","timestamp":1304148401000},"page":"12-19","source":"Crossref","is-referenced-by-count":28,"title":["Modulatory Effects of Bovine Seminal Plasma on Uterine Inflammatory 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