{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T16:25:36Z","timestamp":1791044736137,"version":"4.1.0"},"reference-count":64,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2011,5,20]],"date-time":"2011-05-20T00:00:00Z","timestamp":1305849600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2012,2]]},"abstract":"<jats:title>Contents<\/jats:title><jats:p>This study was performed to determine the effect of intracerebroventricular (icv) injection of interleukin (IL)\u20101\u03b2 on the gene expression, translation and release of gonadotropin\u2010releasing hormone (GnRH) and the GnRH receptor (GnRHR) gene expression in the hypothalamus of anestrous ewes. In the anterior pituitary gland (AP), the expression of genes encoding: GnRHR, \u03b2 subunits of luteinizing hormone (LH) and folliculotropic hormone (FSH) was determined as well as the effect of IL\u20101\u03b2 on pituitary gonadotropins release. The relative mRNA level was determined by real\u2010time PCR, GnRH concentration in the cerebrospinal fluid (CSF) was assayed by ELISA and the plasma concentration of LH and FSH were determined by radioimmunoassay. Our results showed that icv injection of IL\u20101\u03b2 (10 or 50\u2003\u03bcg\/animal) decreased the GnRH mRNA level in the pre\u2010optic area (POA) (35% and 40% respectively; p\u2003\u2264\u20030.01) and median eminence (ME) (75% and 70% respectively; p\u2003\u2264\u20030.01) and GnRHR gene expression in ME (55% and 50% respectively; p\u2003\u2264\u20030.01). A significant decrease in GnRHR mRNA level in the AP in the group treated with the 50\u2003\u03bcg (60%; p\u2003\u2264\u20030.01) but not with the 10\u2003\u03bcg dose was observed. The centrally administrated IL\u20101\u03b2 lowered also GnRH concentration in the CSF (60%; p\u2003\u2264\u20030.01) and reduced the intensity of GnRH translation in the POA (p\u2003\u2264\u20030.01). It was not found any effect of icv IL\u20101\u03b2 injection upon the release of LH and FSH. However, the central injection of IL\u20101\u03b2 strongly decreased the LH\u03b2 mRNA level (41% and 50%; p\u2003\u2264\u20030.01; respectively) and FSH\u03b2 mRNA in the case of the 50\u2003\u03bcg dose (49%; p\u2003\u2264\u20030.01) in the pituitary of anestrous ewes. These results demonstrate that the central IL\u20101\u03b2 is an important modulator of the GnRH biosynthesis and release during immune\/inflammatory challenge.<\/jats:p>","DOI":"10.1111\/j.1439-0531.2011.01800.x","type":"journal-article","created":{"date-parts":[[2011,5,20]],"date-time":"2011-05-20T08:05:22Z","timestamp":1305878722000},"page":"44-52","source":"Crossref","is-referenced-by-count":40,"title":["Central Injection of Exogenous IL\u20101\u03b2 in the Control Activities of Hypothalamic\u2013Pituitary\u2013Gonadal Axis in Anestrous Ewes"],"prefix":"10.1111","volume":"47","author":[{"given":"AP","family":"Herman","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T","family":"Misztal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K","family":"Romanowicz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D","family":"Tomaszewska\u2010Zaremba","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2011,5,20]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"crossref","first-page":"988","DOI":"10.1016\/S0022-3565(25)20576-6","article-title":"Human interleukin (IL) 1\u03b1, murine interleukin\u20101\u03b1 and murine interleukin\u20101\u03b2 are transported from blood to brain in the mouse by a shared saturable mechanism","volume":"259","author":"Banks 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