{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,20]],"date-time":"2026-06-20T15:02:38Z","timestamp":1781967758996,"version":"3.54.5"},"reference-count":47,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2011,5,26]],"date-time":"2011-05-26T00:00:00Z","timestamp":1306368000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2012,2]]},"abstract":"<jats:title>Contents<\/jats:title><jats:p>Artificial insemination (AI) of sows results in a significant elevation of prostaglandin F<jats:sub>2\u03b1<\/jats:sub> metabolite (PGFM) levels in peripheral plasma, whereas in mated sows such elevation is not seen. The aim of this study was to investigate whether boar seminal plasma (SP) has any effect on the release of PGFM, prostaglandin F<jats:sub>2\u03b1<\/jats:sub> (PGF<jats:sub>2\u03b1<\/jats:sub>), prostaglandin E<jats:sub>2<\/jats:sub> (PGE<jats:sub>2<\/jats:sub>) or interleukin\u20106 (IL\u20106) by <jats:italic>in vitro<\/jats:italic> cultured porcine endometrial (epithelial \u2013 pUE and stromal \u2013 pUS), cervical (pCE and pCS) and bovine endometrial epithelial cells (bUE). This study shows that boar SP inhibits the release of PGFM, PGF<jats:sub>2\u03b1<\/jats:sub> and PGE<jats:sub>2<\/jats:sub> by porcine endometrial and cervical cells and bovine endometrial cells after 3 and 24\u2003h incubation. Boar SP stimulated IL\u20106 release by pUE, pUS and even bUE after 3\u2003h incubation. Tumour necrosis factor \u03b1 (TNF\u03b1) stimulated the release of IL\u20106 by pUS only after 24\u2003h incubation, but in the presence of boar SP, this stimulation was attenuated. The overall results from these <jats:italic>in vitro<\/jats:italic> studies give us possibility to understand the difference in prostaglandin response between mated and inseminated sows. Furthermore, we demonstrated that frozen\u2010stored epithelial and stromal cells from pig endometrium, as well as from the cervix are suitable for studying the effect of SP on the release of prostaglandins. The only prerequisite is to incubate these thawed cells with arachidonic acid as a source for the synthesis of prostaglandins. A similar effect of boar SP on porcine and bUE cells may suggest inter\u2010species reactivity.<\/jats:p>","DOI":"10.1111\/j.1439-0531.2011.01809.x","type":"journal-article","created":{"date-parts":[[2011,5,26]],"date-time":"2011-05-26T13:36:42Z","timestamp":1306417002000},"page":"113-124","source":"Crossref","is-referenced-by-count":4,"title":["The Effect of Boar Seminal Plasma on the Release of Prostaglandins and Interleukin\u20106 by Porcine Endometrial and Cervical Cells and Bovine Endometrial 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