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Epididymal sperm recovery was performed using both the float\u2010up (FL) and the retrograde flushing (RF) technique. Within extraction methods, half testes were processed with a Tris egg yolk extender and half with a Tris egg yolk\u2010free extender. Sperm concentration, motility, viability and morphology were evaluated. Sperm concentration was not significantly different between methods. Flushing technique was significantly better than the FL method in terms of sperm quality, considering total motility (80.3\u2003\u00b1\u20032.3% vs 71.6\u2003\u00b1 2.0%, p<jats:italic>\u2003<\/jats:italic>&lt;<jats:italic>\u2003<\/jats:italic>0.001, respectively) and viability (84.5\u2003\u00b1\u20031.5% vs 77.2\u2003\u00b1\u20031.3%, p<jats:italic>\u2003<\/jats:italic>&lt;<jats:italic>\u2003<\/jats:italic>0.001, respectively). Egg yolk influenced positively motility and morphology in the FL method, whereas decreased viability in flushed samples. Results suggest the use of the RF technique to collect cattle epididymal sperm.<\/jats:p>","DOI":"10.1111\/j.1439-0531.2011.01948.x","type":"journal-article","created":{"date-parts":[[2011,11,22]],"date-time":"2011-11-22T13:37:16Z","timestamp":1321969036000},"page":"712-717","source":"Crossref","is-referenced-by-count":22,"title":["Influence of Recovery Methods and Extenders on Bull Epididymal Spermatozoa Quality"],"prefix":"10.1111","volume":"47","author":[{"given":"F","family":"Turri","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Madeddu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"TM","family":"Gliozzi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G","family":"Gandini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F","family":"Pizzi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2011,11,22]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cryobiol.2009.06.009"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(03)00259-0"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1530\/rep.1.00011"},{"key":"e_1_2_8_5_1","first-page":"29","volume-title":"Techniques for Semen Evaluation, Semen Storage, and Fertility Determination","author":"Bakst MR","year":"1997"},{"key":"e_1_2_8_6_1","first-page":"1","volume-title":"Abnormal Morphology of Bovine Spermatozoa","author":"Barth AD","year":"1989"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cveq.2006.08.007"},{"key":"e_1_2_8_8_1","first-page":"35","article-title":"A comparison of electroejaculation and epididymal sperm collection techniques in stallion","volume":"45","author":"Cary JA","year":"2004","journal-title":"Can Vet J"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1939-4640.1997.tb01953.x"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.2164\/jandrol.108.006395"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0531.2006.00649.x"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10344-007-0089-z"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1939-4640.2000.tb03388.x"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0490277"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0600165"},{"key":"e_1_2_8_16_1","unstructured":"FreneauGE PuoliJR BorjaALR 2000:\u00cdndice de capacidade androl\u00f3gica por pontos (ICAP) em touros Nelore: Estudo de esta\u00e7\u00e3o de acasalamento em Mato Grosso do Sul. 2000. 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