{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T00:01:19Z","timestamp":1771632079377,"version":"3.50.1"},"reference-count":51,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2012,2,15]],"date-time":"2012-02-15T00:00:00Z","timestamp":1329264000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2012,12]]},"abstract":"<jats:title>Contents<\/jats:title><jats:p>Glucocorticoids (GCs) are important mediators of the stress response and have been implicated in the function and regulation of testicular functions in different species. In many tissues, intracellular glucocorticoid activity is controlled by either or both of the two known isoforms of 11\u03b2\u2010hydroxysteroid dehydrogenase (11\u03b2HSD) type 1 and 2, which interconvert active and inactive GCs. Little is known about the effects of stress on fertility in the equine species. The main objective of the present study was to investigate the expression of receptors for GCs and adrenocorticotropic hormone [ACTH, melanocortin 2 receptor (MC2R)] as well 11\u03b2HSD1 and 11\u03b2HSD2 in male equine epididymal and testicular tissue. In addition, expression of aromatase P\u2010450 and receptors for luteinizing hormone (LHR), follicle stimulating hormone (FSHR) and growth hormone (GHR) was studied. Reverse transcriptase PCR and quantitative real\u2010time PCR were performed in tissue from the epididymis (caput and cauda) and testes collected from nine healthy mature stallions (age 4\u201310\u2003years). mRNA for ACTH and GC receptors as well as 11\u03b2HSD1 and \u20102 were found in epididymal and testicular tissue. Expression of the genes studied was always positive in testicular tissue, while it was inconsistent in epididymal tissue. Quantitative gene expression in relation to \u03b2\u2010actin and glyceraldehyde\u20103\u2010phosphate dehydrogenase (GAPDH) was significantly correlated (<jats:italic>R<\/jats:italic>\u2003=\u20030.403, p\u2003&lt;\u20030.001). Quantitative PCR in relation to \u03b2\u2010actin revealed significant differences in the gene expression of 11\u03b2HSD1, 11\u03b2HSD2, LHR, FSHR, MC2R and aromatase between tissue collected from caput epididymidis, cauda epididymidis and testicular parenchyma (p\u2003&lt;\u20030.05). With GAPDH, differences between tissues were significant for 11\u03b2HSD1, 11\u03b2HSD2 and MC2R (p\u2003&lt;\u20030.05) In addition, high concentrations of mRNA of aromatase and receptors of LH and FSH were found in testicular tissue, while a pronounced expression of GH receptor was present in epididymal tissue. The results support the hypothesis of an interaction between the pituitary\u2010adrenal axis and testicular function in the stallion.<\/jats:p>","DOI":"10.1111\/j.1439-0531.2012.01993.x","type":"journal-article","created":{"date-parts":[[2012,2,15]],"date-time":"2012-02-15T14:22:31Z","timestamp":1329315751000},"page":"928-935","source":"Crossref","is-referenced-by-count":20,"title":["Gene Expression of ACTH, Glucocorticoid Receptors, 11\u03b2HSD Enzymes, LH\u2010, FSH\u2010, GH Receptors and Aromatase in Equine Epididymal and Testicular 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