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Proteins are one of the major SP components that modulate sperm functionality. During the last years, intensive work has been performed to characterize the role of these proteins. They have been found to influence sperm capacitation, formation of the oviductal sperm reservoir and sperm\u2013oocyte interaction. Sperm biotechnologies, such as sperm cryopreservation and flow cytometric sex\u2010sorting, that involve a substantial dilution of the SP are detrimental to sperm quality. Attempts to improve the outcome of these biotechnologies include the restoration of SP, which has produced contradictory results. To overcome this variability, different research groups have proposed the application of isolated SP proteins. Herein, we will review the current knowledge in the role of the major SP proteins as modulators of sperm functionality. Furthermore, we will discuss the possible applications of the SP proteins in sperm cryopreservation and flow cytometric sex\u2010sorting.<\/jats:p>","DOI":"10.1111\/j.1439-0531.2012.02028.x","type":"journal-article","created":{"date-parts":[[2012,6,11]],"date-time":"2012-06-11T06:46:28Z","timestamp":1339397188000},"page":"12-21","source":"Crossref","is-referenced-by-count":104,"title":["Seminal Plasma Proteins as Modulators of the Sperm Function and Their Application in Sperm Biotechnologies"],"prefix":"10.1111","volume":"47","author":[{"given":"I","family":"Caballero","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I","family":"Parrilla","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C","family":"Almi\u00f1ana","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D","family":"del Olmo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J","family":"Roca","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"EA","family":"Mart\u00ednez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"JM","family":"V\u00e1zquez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,6,11]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1071\/RD9930361"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0025993"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1071\/RD9940173"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.102.007013"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(96)00237-3"},{"key":"e_1_2_7_7_1","unstructured":"Azer\u00eadoGA EsperCR ResendeKT 2000:Use of fluorescent probes in the evaluation of plasma membrane integrity of frozen\u2010thawed goat spermatozoa with or without seminal plasma. 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