{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,29]],"date-time":"2023-10-29T04:31:43Z","timestamp":1698553903798},"reference-count":17,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2012,5,21]],"date-time":"2012-05-21T00:00:00Z","timestamp":1337558400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2013,4]]},"abstract":"<jats:title>Contents<\/jats:title><jats:p>A new device for storage and shipping of cell cultures \u2013 the Petaka G3 cell management device \u2013 was tested for its applicability for cooled\u2010storage of equine semen. Semen from three stallions was processed with EquiPro extender either without antibiotics (three ejaculates per stallion) or with gentamicin (250\u2003mg\u200a\/\u200al; three ejaculates per stallion). Semen was either stored at five (anaerobic conditions) or 15\u00b0C (aerobic conditions) in syringes or cell culture devices. Total and progressive motility, as well as membrane integrity of spermatozoa, were evaluated from days 1 to 7 after collection with computer\u2010assisted semen analysis. In experiment 1 (extender without antibiotics), total motility, progressive motility and viability of spermatozoa significantly decreased over time (p\u2003&lt;\u20030.05). The decrease was significantly faster at 15\u00b0C than at 5\u00b0C (p\u2003&lt;\u20030.05). In the presence of gentamicin (experiment 2), this difference was no longer present. It can be concluded that cooled\u2010storage of equine semen in sophisticated devices for cell culture is not advantageous to syringes for successful maintenance of semen longevity.<\/jats:p>","DOI":"10.1111\/j.1439-0531.2012.02115.x","type":"journal-article","created":{"date-parts":[[2012,5,21]],"date-time":"2012-05-21T13:43:35Z","timestamp":1337607815000},"source":"Crossref","is-referenced-by-count":0,"title":["Applicability of a New Cell Culture Device for Cooled\u2010Storage of Stallion Semen"],"prefix":"10.1111","volume":"48","author":[{"given":"D","family":"Scarlet","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S","family":"Budik","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C","family":"Aurich","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,5,21]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1071\/RD9940019"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2008.04.015"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2006.11.004"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(97)00250-1"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(00)00304-6"},{"key":"e_1_2_8_7_1","doi-asserted-by":"crossref","first-page":"12","DOI":"10.2527\/2002.80112x","article-title":"Effect of pyruvate on the function of stallion spermatozoa stored for up to 48\u2003h","volume":"80","author":"Bruemmert JE","year":"2002","journal-title":"J Anim Sci"},{"key":"e_1_2_8_8_1","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1002\/j.1939-4640.1992.tb03328.x","article-title":"Reactive oxygen species and human spermatozoa II. 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