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The proestrous\u2010 and oestrous\u2010stage mucus proteins were purified and analysed with proteomic tools such as two\u2010dimensional gel electrophoresis and matrix\u2010assisted laser desorption\/ionization\u2013time\u2010of\u2010flight analyses. Among the differentially expressed proteins, the dimethylarginine dimethylaminohydrolase 2 (DDAH2) protein showed a 3.6\u2010fold increase during the proestrous stage compared to that during the oestrous stage. A western immunoblot study revealed that two of three sow mucus samples clearly showed negative anti\u2010DDAH2 antibody activity during the oestrous stage. This study demonstrated that the pig DDAH2 mucus protein exists during the proestrous stage, but not during the oestrous stage, suggesting that mucus DDAH2 could be useful as an oestrus detection marker.<\/jats:p>","DOI":"10.1111\/j.1439-0531.2012.02122.x","type":"journal-article","created":{"date-parts":[[2012,6,27]],"date-time":"2012-06-27T17:30:56Z","timestamp":1340818256000},"source":"Crossref","is-referenced-by-count":1,"title":["Identification of the DDAH2 Protein in Pig Reproductive Tract Mucus: A Putative Oestrus Detection Marker"],"prefix":"10.1111","volume":"48","author":[{"given":"WY","family":"Lee","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"SY","family":"Chai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"KH","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"HJ","family":"Park","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"JH","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"NH","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"HS","family":"Jeon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"IC","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"HS","family":"Choi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H","family":"Song","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,6,27]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(99)00029-1"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.2527\/1998.7692235x"},{"key":"e_1_2_8_4_1","first-page":"619","article-title":"Proliferative changes in the genital tissue of female mice during the oestrous cycle","volume":"23","author":"Evans GS","year":"1990","journal-title":"Cell Tissue Kinet"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod21.3.617"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.S0022-0302(76)84307-X"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1997.83.6.1785"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200600124"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1996.tb16069.x"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/gah072"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/aja.2008.54"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.2527\/jas.2008-1181"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(01)00663-X"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0150307"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9378(11)91597-X"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1071-5576(97)00106-8"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2008.08.005"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2007.10.002"}],"container-title":["Reproduction in Domestic Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1439-0531.2012.02122.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1439-0531.2012.02122.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,28]],"date-time":"2023-10-28T20:21:28Z","timestamp":1698524488000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1439-0531.2012.02122.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,6,27]]},"references-count":17,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2013,2]]}},"alternative-id":["10.1111\/j.1439-0531.2012.02122.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1439-0531.2012.02122.x","archive":["Portico"],"relation":{},"ISSN":["0936-6768","1439-0531"],"issn-type":[{"value":"0936-6768","type":"print"},{"value":"1439-0531","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,6,27]]}}}