{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T03:33:26Z","timestamp":1761708806806},"reference-count":28,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2012,8,9]],"date-time":"2012-08-09T00:00:00Z","timestamp":1344470400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2013,4]]},"abstract":"<jats:title>Contents<\/jats:title><jats:p>Real\u2010time PCR was used to investigate the role of progesterone (P4) and oestradiol (E2) in regulation of endometrial cytosolic, secretory and calcium\u2010independent phospholipase A2 (<jats:italic>PLA2G4A<\/jats:italic>, <jats:italic>PLA2G2A<\/jats:italic> and <jats:italic>PLA2G6<\/jats:italic>, respectively) gene expression. Ovariectomized mares underwent 6\u2003days of E2 pre\u2010treatment followed by 14\u2003days of P4 supplementation. At the start of P4 treatment (Day 1), mares were assigned in a 2\u2003\u00d7\u20032 factorial design to receive either E2 or vehicle starting on Day 11 and endometrial biopsy collection on either Day 14 when P4 concentrations remained high (&gt;4\u2003ng\/ml) or Day 16 when P4 concentrations had declined (0.5\u20132\u2003ng\/ml). Additional biopsies were collected from ovariectomized mares on Day 8, which served as control. Blood samples were collected for P4 determination. <jats:italic>PLA2G4A<\/jats:italic> expression was higher (p\u2003&lt;\u20030.05) on Day 14 compared with Day 8. In contrast, <jats:italic>PLA2G2A<\/jats:italic> did not change significantly (p\u2003&lt;\u20030.12). <jats:italic>PLA2G4A<\/jats:italic> and <jats:italic>PLA2G2A<\/jats:italic> gene expression increased (p\u2003&lt;\u20030.05), as P4 concentration dropped, on Day 16. In contrast, <jats:italic>PLA2G6<\/jats:italic> gene expression did not show differences between days. Treatment with oestradiol did not increase <jats:italic>PLA2<\/jats:italic> isoforms expression when compared to treatment with the vehicle. <jats:italic>PLA2G4A<\/jats:italic> and <jats:italic>PLA2G2A<\/jats:italic> were positively correlated with each other and negatively correlated with P4 concentrations. In conclusion, P4 withdrawal upregulated <jats:italic>PLA2G4A<\/jats:italic> and <jats:italic>PLA2G2A<\/jats:italic> gene expression, and this was not affected by E2. <jats:italic>PLA2G4A<\/jats:italic> and <jats:italic>PLA2G2A<\/jats:italic> but not <jats:italic>PLA2G6<\/jats:italic> gene expression may be involved in controlling prostaglandin F2 alpha synthesis and luteolysis.<\/jats:p>","DOI":"10.1111\/j.1439-0531.2012.02151.x","type":"journal-article","created":{"date-parts":[[2012,8,9]],"date-time":"2012-08-09T13:21:23Z","timestamp":1344518483000},"page":"311-316","source":"Crossref","is-referenced-by-count":14,"title":["Ovarian Steroid Regulation of Endometrial Phospholipase A2 Isoforms in 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