{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T00:57:07Z","timestamp":1787014627640,"version":"build-2736575974"},"reference-count":48,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2012,7,27]],"date-time":"2012-07-27T00:00:00Z","timestamp":1343347200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2013,4]]},"abstract":"<jats:title>Contents<\/jats:title>\n                  <jats:p>\n                    Flow cytometry is a useful tool that provides an accurate, objective and rapid evaluation of semen quality. The use of this technique could significantly improve the quality of buffalo semen samples used in artificial insemination. This study was carried out to evaluate, by flow cytometry, frozen\u2013thawed buffalo spermatozoa quality parameters such as sperm viability by SYBR\u201014\/propidium iodide staining; mitochondrial function by JC\u20101 potentiometric probe; sperm chromatin stability (SCSA) by acridine orange; and acrosome reaction (AR) by FITC\u2010PNA staining. Semen samples from five Italian Mediterranean buffalo bulls were used. Sperm viability was not different between bulls and ranged from 33.4% to 43.6%. A consistent rate (55.1\u2003\u00b1\u200310.8%) of sperm cells showed high mitochondrial membrane potential (\u0394\u03c8\n                    <jats:sup>high<\/jats:sup>\n                    ), with no significant differences between subjects. Sperm chromatin structure assay differed significantly between the five buffalo bulls; moreover, data showed high stability within each buffalo. DNA fragmentation indexes (DFI), such as %\u2010DFI,\n                    <jats:inline-graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"graphic\/rda2152_mu1.gif\" xlink:title=\"inline image\"\/>\n                    \u2010DFI, SD\u2010DFI, were 11.2\u2003\u00b1\u20038.6, 153.3\u2003\u00b1\u200324.6 and 81.6\u2003\u00b1\u200321.2, respectively. Regarding AR, the percentage of acrosome\u2010reacted live (ARL) and acrosome\u2010reacted dead (ARD) spermatozoa was 0.3\u2003\u00b1\u20030.2 and 15.3\u2003\u00b1\u20035.5, respectively. This functional parameter differed significantly between buffalo bulls and showed high stability. Following to Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ionophore A23187 for 3\u2003h, AR significantly differed between subjects and was characterized by an increase in both ARL (10.8%) and ARD population (22.0%). This study indicates that flow cytometry could be a useful tool for a quick multiparametric evaluation of sperm quality in buffalo. In particular, SCSA and AR resulted in sperm functional parameters sensitive enough for the diagnosis of frozen\u2010thawed semen fertilizing potential.\n                  <\/jats:p>","DOI":"10.1111\/j.1439-0531.2012.02152.x","type":"journal-article","created":{"date-parts":[[2012,7,27]],"date-time":"2012-07-27T11:19:55Z","timestamp":1343387995000},"page":"317-324","source":"Crossref","is-referenced-by-count":30,"title":["Assessment of Different Functional Parameters of Frozen\u2013Thawed Buffalo Spermatozoa by Using Cytofluorimetric 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