{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,3]],"date-time":"2025-10-03T22:18:05Z","timestamp":1759529885080},"reference-count":50,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2004,5,18]],"date-time":"2004-05-18T00:00:00Z","timestamp":1084838400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Gastro and Hepatol"],"published-print":{"date-parts":[[2004,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:bold>Background and Aim:\u2002<\/jats:bold> The present study was designed to investigate the chemopreventive effects of combined vanadium (V; 0.5\u2003p.p.m.) and beta\u2010carotene (BC; 120\u2003mg\/kg of basal diet) on diethylnitrosoamine (DEN)\u2010induced and phenobarbital (PB)\u2010promoted rat hepatocarcinogenesis.<\/jats:p><jats:p><jats:bold>Methods:\u2002<\/jats:bold> All rats were subjected to two\u2010thirds partial hepatectomy (PH) at the fourth week. After PH they were administered either trioctanoin alone (groups A\u2032, B\u2032, C\u2032 and D\u2032) or a single injection of DEN in trioctanoin at a dose of 10\u2003mg\/kg of body weight (groups A, B, C and D). Two weeks after the DEN treatment PB was administered (0.05% in basal diet) to all the DEN\u2010treated rats and continued until the end of the experiment. Supplementation of V (groups B and B\u2032), BC (groups C and C\u2032) or both V and BC (groups D and D\u2032) at the doses stated previously were started 4\u2003weeks before DEN administration (at week\u20030) and continued until the 16th week.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> It was observed that in the DEN\u2010treated and PB\u2010promoted group (group A) the expression of the numbers and areas of the placental form of glutathione S\u2010transferase (GST\u2010P)\u2010positive altered hepatic foci (AHF) was maximum. Treatment with V (group B) and BC (group C) significantly reduced the expression of GST\u2010P\u2010positive AHF by 29.5% and 42.8%, respectively. An additive protection action (65.7%) was noticed in group D, which received both V and BC for the entire period of the experiment. It was also observed that supplementation of V and BC for the entire period of the experiment significantly reduced the number and size of the hyperplastic nodules, while the combination treatment worked as an additive effect, reducing the number and size of the hyperplastic nodules to 22% from 89%. Moreover, a significantly reduced level of cytosolic glutathione (<jats:italic>P<\/jats:italic>\u2003&lt;\u20030.001) and glutathione\u2010S\u2010transferase (<jats:italic>P<\/jats:italic>\u2003&lt;\u20030.001) activity and stabilization of aerobic metabolism and hepatic architecture of the cells as compared with carcinogen control were observed in the V\u2003+\u2003BC\u2010treated group.<\/jats:p><jats:p><jats:bold>Conclusion:\u2002<\/jats:bold> The present study suggests that V, an essential trace element, may be useful in combination with BC, an antioxidant, in the inhibition of experimentally induced rat hepatocarcinogenesis.<\/jats:p>","DOI":"10.1111\/j.1440-1746.2004.03378.x","type":"journal-article","created":{"date-parts":[[2004,12,27]],"date-time":"2004-12-27T07:32:17Z","timestamp":1104132737000},"page":"683-693","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Combined supplementation of vanadium and beta\u2010carotene suppresses placental glutathione S\u2010transferase\u2010positive foci and enhances antioxidant functions during the inhibition of diethylnitrosamine\u2010induced rat liver carcinogenesis"],"prefix":"10.1111","volume":"19","author":[{"given":"MITALI BASU","family":"CHATTOPADHYAY","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"MAHENDRA KUMAR","family":"CB","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P SURESH","family":"KANNA","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"RAJARSHI SANKAR","family":"RAY","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"SOUVIK","family":"ROY","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"MALAY","family":"CHATTERJEE","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,5,18]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0304-419X(80)90002-5"},{"key":"e_1_2_6_3_2","first-page":"23","article-title":"Changes in morphology of induced liver cancer treated with Strobilanthes crispus","volume":"1","author":"Elizabeth M","year":"2000","journal-title":"Anal. 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