{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,1,23]],"date-time":"2024-01-23T14:40:17Z","timestamp":1706020817579},"reference-count":30,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2004,10,8]],"date-time":"2004-10-08T00:00:00Z","timestamp":1097193600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Gastro and Hepatol"],"published-print":{"date-parts":[[2004,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:bold>Background and Aims:\u2002<\/jats:bold>  <jats:italic>Helicobacter pylori<\/jats:italic> infection is linked with increased antralization at the gastric incisura. The present study aimed to determine if antralization is associated with altered gastric epithelial apoptosis and proliferation and with seropositivity of the cytotoxin\u2010associated gene product A (CagA) of <jats:italic>H.\u2003pylori<\/jats:italic>.<\/jats:p><jats:p><jats:bold>Methods:\u2002<\/jats:bold> Gastric biopsies taken from the antrum, incisura, body and fundus of 75 patients (34 male, 41 female; mean age 59.5\u2003years) were used for diagnosis of <jats:italic>H.\u2003pylori<\/jats:italic> infection and assessments of histological changes. Apoptosis and Ki\u201067 expression in epithelial cells were determined for the antral, incisura and body biopsies by immunohistochemistry. Serum samples were tested by enzyme\u2010linked immunosorbent assays for anti\u2010<jats:italic>H.\u2003pylori<\/jats:italic> and anti\u2010CagA IgG antibodies.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> Apoptotic index (AI) and Ki\u201067 proliferation index (PI) were greater in the presence (<jats:italic>vs<\/jats:italic> absence) of <jats:italic>H.\u2003pylori<\/jats:italic> infection at the antrum, incisura and body (all <jats:italic>P<\/jats:italic>\u2003&lt;\u20030.001), and topographically associated with chronic gastritis and gastric atrophy\/intestinal metaplasia at the antrum and incisura (all <jats:italic>P<\/jats:italic>\u2003&lt;\u20030.001). Moreover, AI and PI were greater in the presence (<jats:italic>vs<\/jats:italic> absence) of antralization at the incisura (20.2\u2003\u00b1\u20030.9 <jats:italic>vs<\/jats:italic> 11.4\u2003\u00b1\u20030.1.1 and 48.9\u2003\u00b1\u20032.5 <jats:italic>vs<\/jats:italic> 29.9\u2003\u00b1\u20032.5, both <jats:italic>P<\/jats:italic>\u2003&lt;\u20030.001). CagA seroprevalence was 41% in the 39 infected patients. CagA seropositivity was associated with gastric atrophy\/intestinal metaplasia at the antrum (\u03c7<jats:sup>2<\/jats:sup>\u2003=\u20034.67, <jats:italic>P<\/jats:italic>\u2003=\u20030.03) and incisura (\u03c7<jats:sup>2<\/jats:sup>\u2003=\u20034.88, <jats:italic>P<\/jats:italic>\u2003=\u20030.03), but not associated with gastric epithelial apoptosis and Ki\u201067 expression, nor with antralization at the incisura.<\/jats:p><jats:p><jats:bold>Conclusions:\u2002<\/jats:bold> Antralization of gastric incisura is topographically associated with increased gastric epithelial apoptosis and proliferation, but not with CagA seropositivity.<\/jats:p>","DOI":"10.1111\/j.1440-1746.2004.03489.x","type":"journal-article","created":{"date-parts":[[2004,10,8]],"date-time":"2004-10-08T14:49:14Z","timestamp":1097246954000},"page":"1257-1263","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Antralization of gastric incisura is topographically associated with increased gastric epithelial apoptosis and proliferation, but not with CagA seropositivity"],"prefix":"10.1111","volume":"19","author":[{"given":"HARRY HUA\u2010XIANG","family":"XIA","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"BENJAMIN CHUN\u2010YU","family":"WONG","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"GUI\u2010SHUI","family":"ZHANG","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"YI","family":"YANG","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"JENNY MA","family":"WYATT","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"STUART","family":"ADAMS","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"KAREN","family":"CHEUNG","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"SHIU KUM","family":"LAM","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"NICHOLAS J","family":"TALLEY","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,10,8]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1572-0241.1999.01360.x"},{"key":"e_1_2_6_3_2","first-page":"4255","article-title":"Development of Helicobacter pylori\u2010induced gastric carcinoma in Mongolian gerbils","volume":"58","author":"Honda S","year":"1998","journal-title":"Cancer Res."},{"key":"e_1_2_6_4_2","first-page":"217","article-title":"Helicobacter pylori infection and gastric cancer","volume":"24","author":"Xia HHX","year":"2001","journal-title":"Asian J. Surg."},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.3109\/00365529409092480"},{"key":"e_1_2_6_6_2","first-page":"1317","article-title":"Precancerous gastric lesions in a population at high risk of stomach cancer","volume":"53","author":"You WC","year":"1993","journal-title":"Cancer Res."},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1097\/00000478-199610000-00001"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1572-0241.2000.01609.x"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1572-0241.2002.07120.x"},{"key":"e_1_2_6_10_2","first-page":"7415","article-title":"Increased cell division as a cause of human cancer","volume":"50","author":"Preston\u2010Martin S","year":"1990","journal-title":"Cancer Res."},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9270(00)02240-1"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1136\/gut.38.4.498"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02212681"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1018830408397"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1136\/mp.56.1.19"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-5378.2001.00040.x"},{"key":"e_1_2_6_17_2","doi-asserted-by":"crossref","first-page":"14648","DOI":"10.1073\/pnas.93.25.14648","article-title":"Cag, pathogenicity island of Helicobacter pylori, encodes type I\u2010specific and disease associated virulence factors","volume":"93","author":"Censini S","year":"1996","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"e_1_2_6_18_2","first-page":"6124","article-title":"Helicobacter pylori strain\u2010specific genotypes and modulations of the gastric epithelial cell cycle","volume":"59","author":"Peek RM","year":"1999","journal-title":"Cancer Res."},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/89.12.863"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1026636803101"},{"key":"e_1_2_6_21_2","first-page":"1406","article-title":"Increased gastric epithelial cell apoptosis associated with colonization with cagA+ Helicobacter pylori strains","volume":"61","author":"Moss SF","year":"2001","journal-title":"Cancer Res."},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0016-5085(97)70003-9"},{"key":"e_1_2_6_23_2","first-page":"2277","article-title":"Helicobacter pylori inhibits the G(1) to S transition in AGS gastric epithelial cells","volume":"59","author":"Shirin AH","year":"1999","journal-title":"Cancer Res."},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1015069519610"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1007\/s005350070003"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0732-8893(99)00101-7"},{"key":"e_1_2_6_27_2","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1002\/(SICI)1097-0215(20000115)85:2%3C192::AID-IJC8%3E3.0.CO;2-F","article-title":"Altered gastric epithelial cell kinetics in Helicobacter pylori\u2010associated intestinal metaplasia: implications for gastric carcinogenesis","volume":"85","author":"Scotiniotis IA","year":"2000","journal-title":"Int. J. Cancer"},{"key":"e_1_2_6_28_2","first-page":"1695","article-title":"Increase in proliferation and apoptosis of gastric epithelial cells early in the natural history of Helicobacter pylori infection","volume":"151","author":"Jones NL","year":"1997","journal-title":"Am. J. Pathol."},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1136\/gut.44.6.789"},{"key":"e_1_2_6_30_2","doi-asserted-by":"crossref","first-page":"584","DOI":"10.1016\/S0016-5085(19)33241-X","article-title":"Chronological transition of the fundic pyloric border determined by stepwise biopsy of the lesser and greater curvatures of the stomach","volume":"63","author":"Kimura K","year":"1972","journal-title":"Gastroenterology"},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v9.i6.1265"}],"container-title":["Journal of Gastroenterology and Hepatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1440-1746.2004.03489.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1440-1746.2004.03489.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,10]],"date-time":"2023-10-10T01:24:09Z","timestamp":1696901049000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1440-1746.2004.03489.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,10,8]]},"references-count":30,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2004,11]]}},"alternative-id":["10.1111\/j.1440-1746.2004.03489.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1440-1746.2004.03489.x","archive":["Portico"],"relation":{},"ISSN":["0815-9319","1440-1746"],"issn-type":[{"value":"0815-9319","type":"print"},{"value":"1440-1746","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,10,8]]},"assertion":[{"value":"2004-10-08","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}