{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T16:32:18Z","timestamp":1789576338443,"version":"build-2803163510"},"reference-count":39,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2005,4,15]],"date-time":"2005-04-15T00:00:00Z","timestamp":1113523200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Gastro and Hepatol"],"published-print":{"date-parts":[[2005,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:bold>Background:<\/jats:bold> The pathophysiology of constipation is not clearly identified as yet, and the interstital cells of Cajal (ICC), known to generate the slow wave activity and to be involved in intestinal neurotransmission and the enteric nervous system (ENS), are suspected to play an important role. The aims of the present study were to assess the distribution of ICC and neuronal cells of ENS in patients with slow\u2010transit constipation and acquired megacolon.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> Sigmoid colon specimens were obtained from patients who underwent colectomy due to slow\u2010transit constipation (<jats:italic>n<\/jats:italic>\u2003=\u200310), acquired megacolon (<jats:italic>n<\/jats:italic>\u2003=\u20039) and non\u2010obstructive colon cancer (<jats:italic>n<\/jats:italic>\u2003=\u200310) as a control group. The ICC were visualized by <jats:italic>c\u2010Kit<\/jats:italic> immunohistochemistry and neuronal cells of the ENS were demonstrated by protein gene product (PGP) 9.5. Density of cells stained by <jats:italic>c\u2010Kit<\/jats:italic> and PGP 9.5 was calculated as percent area (area stained\/area of X\u2013Y plane)\u2003\u00d7\u2003100, when images were collected at a magnification of \u00d740 objective, with maximum area examined in the horizontal X\u2013Y plane of 400\u2003\u00b5m\u2003\u00d7\u2003400\u2003\u00b5m using an image analyzer.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> The densities of ICC and PGP 9.5 reactive neuronal structures were significantly decreased in all layers of sigmoid colon specimens in patients with slow\u2010transit constipation and acquired megacolon, compared with that of the control group. However, there was no statistically significant difference in either the density of ICC or that of neuronal structures between the patients with slow\u2010transit constipation and acquired megacolon.<\/jats:p><jats:p><jats:bold>Conclusions:<\/jats:bold> Slow\u2010transit constipation and acquired megacolon were associated with alteration of ICC and neuronal cells of ENS in the sigmoid colon.<\/jats:p><jats:p>\u00a9 2005 Blackwell Publishing Asia Pty Ltd<\/jats:p>","DOI":"10.1111\/j.1440-1746.2005.03809.x","type":"journal-article","created":{"date-parts":[[2005,7,1]],"date-time":"2005-07-01T17:36:07Z","timestamp":1120239367000},"page":"1292-1298","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":78,"title":["Decreased density of interstitial cells of Cajal and neuronal cells in patients with slow\u2010transit constipation and acquired megacolon"],"prefix":"10.1111","volume":"20","author":[{"given":"JUNG IL","family":"LEE","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"HYOJIN","family":"PARK","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"MICHAEL A","family":"KAMM","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"IAN C","family":"TALBOT","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,7]]},"reference":[{"key":"e_1_2_5_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0016-5085(00)70409-4"},{"key":"e_1_2_5_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0016-5085(85)80128-1"},{"key":"e_1_2_5_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02052258"},{"key":"e_1_2_5_5_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2982.1997.d01-46.x"},{"key":"e_1_2_5_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02063253"},{"key":"e_1_2_5_7_2","doi-asserted-by":"publisher","DOI":"10.1097\/00042737-199612000-00014"},{"key":"e_1_2_5_8_2","first-page":"414","article-title":"A clinical study of some common anatomical abnormalities of the colon. 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