{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,9,16]],"date-time":"2024-09-16T09:12:06Z","timestamp":1726477926059},"reference-count":43,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2006,7,5]],"date-time":"2006-07-05T00:00:00Z","timestamp":1152057600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Gastro and Hepatol"],"published-print":{"date-parts":[[2007,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:bold>Background and Aim:\u2002<\/jats:bold> Portal hypertension is associated with decreased vascular responsiveness to vasoconstrictors, which may contribute to the hyperdynamic circulation in cirrhosis. Animal models of cirrhosis and portal vein ligation have helped in our understanding of portal hypertension. The etiopathogenesis of non\u2010cirrhotic portal fibrosis (NCPF), a common cause of portal hypertension, is still poorly understood. The aim of this study was to investigate the pathophysiology of NCPF in a rabbit model.<\/jats:p><jats:p><jats:bold>Methods:\u2002<\/jats:bold> An indwelling cannula was inserted into the gastrosplenic vein of rabbits. Animals were randomly injected with saline (Group I, <jats:italic>n<\/jats:italic>\u2003=\u200313) or lipopolysaccharide (Group II, <jats:italic>n<\/jats:italic>\u2003=\u200313) from heat killed <jats:italic>Escherichia\u2003coli<\/jats:italic> at 0, 1, 2, 7, 14 and 28\u2003days. Portal pressure was measured at 3\u2003months and vasoresponsiveness studied in isolated aortic rings in intact and in endothelium\u2010denuded tissues from both groups.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> In all group II compared with group I animals, the splenic weight (0.89\u2003\u00b1\u20030.16 <jats:italic>vs<\/jats:italic> 0.62\u2003\u00b1\u20030.1\u2003g, <jats:italic>P<\/jats:italic>\u2003&lt;\u20030.05) and the portal pressure (14.99\u2003\u00b1\u20030.56 <jats:italic>vs<\/jats:italic> 7.04\u2003\u00b1\u20030.42\u2003mmHg, <jats:italic>P<\/jats:italic>\u2003&lt;\u20030.05) were higher at 3\u2003months. The group II animals showed reduced responsiveness to phenylephrine showing maximal contraction of 1.25\u2003\u00b1\u20030.08 at 10<jats:sup>\u22124<\/jats:sup>\u2003mol\/L as compared to 2.85\u2003\u00b1\u20030.33\u2003g tension in Group I (<jats:italic>P<\/jats:italic>\u2003&lt;\u20030.05). Endothelium denudation of aortic rings had no effect on reduced reactivity in Group II animals. Acetylcholine induced an increase in vasorelaxation at lower concentrations in preconstricted aortic rings in Group II compared to Group I animals, but this decreased in higher concentrations. Nifedipine produced comparable vasodilatation in preconstricted rings in both the groups of animals.<\/jats:p><jats:p><jats:bold>Conclusions:\u2002<\/jats:bold> Repeated injection of lipopolysaccharide into the gastrosplenic vein leads to the development of portal hypertension. This non\u2010cirrhotic model of portal hypertension is characterized by generalized arterial hyporeactivity to vasoconstrictors akin to other models of portal hypertension.<\/jats:p>","DOI":"10.1111\/j.1440-1746.2006.04440.x","type":"journal-article","created":{"date-parts":[[2006,7,7]],"date-time":"2006-07-07T05:52:21Z","timestamp":1152251541000},"page":"870-876","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Altered alpha adrenergic vasoresponsiveness in a non\u2010cirrhotic portal hypertension model of <i>E.\u2003coli<\/i> injection"],"prefix":"10.1111","volume":"22","author":[{"given":"Moattar Raza","family":"Rizvi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Swati","family":"Omanwar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohammad","family":"Fahim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shiv Kumar","family":"Sarin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,5]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1136\/gut.30.3.406"},{"key":"e_1_2_6_3_2","doi-asserted-by":"crossref","first-page":"600","DOI":"10.1016\/S0016-5085(84)80106-7","article-title":"Clinical study of 86 cases of idiopathic portal hypertension and comparison with cirrhosis and splenomegaly","volume":"86","author":"Okuda K","year":"1984","journal-title":"Gastroenterology"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/hep.1840110625"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-8278(98)80292-4"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1440-1746.2002.02882.x"},{"key":"e_1_2_6_7_2","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/S0021-9258(19)52451-6","article-title":"Protein measurement with Folin and Ciocalteu\u2019s phenol reagent","volume":"193","author":"Lowry OH","year":"1951","journal-title":"J. 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